Low-flow columns and accessories (Chromatography consumables catalog)

Significance of the topic

Chromatography consumables are critical for achieving reliable, high-sensitivity separations in applications ranging from proteomics and biopharma to environmental and clinical analysis. High-quality sample preparation kits, precision vials, closures, and specialized columns minimize dead volume and system variability, ensuring reproducibility, extended column life, and increased throughput.

Goals and overview of the application note

• Present a connected portfolio of Thermo Scientific chromatography consumables covering sample preparation, sample handling, low-flow LC, BioLC, analytical LC, and GC solutions.
• Demonstrate how integrated column/emitter designs and fingertight fittings simplify nano and capillary LC-MS workflows.
• Introduce product selection tools and resources to guide users in selecting optimal consumables for specific analytical needs.

Methodology and used instrumentation

• Sample preparation products: SMART Digest kits, SOLA SPE, HyperSep SPE, QuEChERS, TurboFlow and μSPE for automated online extraction.
• Sample handling solutions: SureSTART glass vials, AVCS closures, WebSeal well plates, all with low extractables and certified purity.
• Low-flow LC columns and accessories: EASY-Spray, Double nanoViper, and µPAC pillar array columns for bottom-up and top-down proteomics.
• BioLC columns: MAbPac size-exclusion and reversed-phase chemistries for intact proteins, mAbs, and glycan analysis.
• Analytical LC columns: Accucore, Acclaim, Hypersil GOLD, Hypercarb for UHPLC/HPLC separations.
• GC columns and accessories: TracePLOT, TraceGOLD capillary and packed columns, liners, filters, gas management tools.
• Instrumentation examples: Vanquish Neo UHPLC coupled to Orbitrap Exploris 480 MS; EASY-Spray and Flex nanospray sources.

Main results and discussion

• Low-flow LC-MS workflows achieved up to 60% reduction in analysis time without loss of sensitivity using EASY-Spray and PepMap Neo columns.
• Double nanoViper PepMap Neo columns delivered consistent peptide and protein identifications across replicate runs, enabling robust bottom-up proteomics.
• µPAC pillar array columns provided ultrahigh peak capacity, narrow peak widths, and exceptional retention time stability for high-throughput proteome profiling.
• MAbPac capillary reversed-phase columns facilitated accurate measurement of site occupancy and intact mAb characterization in top-down studies.
• Advanced sample preparation kits improved reproducibility, reduced manual handling, and preserved analyte recovery.

Benefits and practical applications

• Streamlined workflows with integrated traps and zero-dead-volume connections reduce setup time and sample loss.
• Enhanced reproducibility with certified low-adsorption vials and low-leachable closures.
• Versatile consumables support a broad range of analytes, from small molecules and oligonucleotides to peptides and monoclonal antibodies.
• Seamless compatibility with existing LC-MS and GC instrument platforms accelerates adoption in R&D and QA/QC laboratories.

Future trends and applications

• Further integration of cloud-based apps and AI-driven method selection will optimize consumable choices and workflows.
• Novel stationary phases and microfabricated devices will push peak capacity and resolution to new limits.
• Miniaturization and automation will enable single-cell and spatial omics analyses with minimal sample volumes.
• Greater focus on sustainable materials and reduced solvent consumption will support green analytical chemistry initiatives.

Conclusion

Thermo Scientific’s connected chromatography consumables deliver high performance, ease of use, and reproducibility across the entire workflow from sample preparation to detection. These solutions empower scientists to accelerate discovery, maintain rigorous quality control, and achieve reliable results in diverse analytical applications.

References

No specific literature references were provided in the original document.