CLAM-2040 - Fully Automated Sample Preparation Module for LC-M

Significance of Automated Sample Preparation for LC–MS in Clinical Research

Liquid chromatography–mass spectrometry (LC–MS) offers unmatched specificity, accuracy and multiplex capability for clinical research. Automating sample preparation removes manual bottlenecks, minimizes errors and enhances reproducibility, enabling laboratories to meet the growing demand for high-throughput bioanalysis.

Objectives and Overview of the Module

The CLAM-2040 is designed as a fully automated, online sample preparation module that integrates seamlessly with LC–MS instruments. Its goal is to simplify workflows, support 24/7 operation, standardize procedures and deliver rapid results with minimal user intervention.

Methodology and Instrumentation

The CLAM-2040 performs all key sample preparation steps automatically: dispensing samples and solvents, adding reagents, shaking and heating, filtering and transferring extracts into the LC–MS autosampler.

Instrumentation used:

• CLAM-2040 automated sample preparation module
• Shimadzu LC–MS systems (triple quadrupoles LCMS-8040, 8050, 8060RX; time-of-flight QTOF LCMS-9030, 9050)
• Specialized single-use extraction filter vials and specialized filter waste container
• Dedicated sample and reagent probes with multiple rinsing solvents
• Barcode reader supporting Code 39, NW-7, Code 128, 2 OF 5

Main Results and Discussion

In optimized conditions, the CLAM-2040 achieves a sample extraction cycle of about 4 minutes and LC–MS analysis of 2.6 minutes, enabling up to 23 results per hour. By preparing up to three samples in parallel and allowing continuous sample loading with priority handling, the system reduces turnaround from the typical 12–60 minutes per result in manual workflows to just 2.6 minutes. Built-in quality control alerts and specification checks ensure consistent performance and data integrity over time.

Benefits and Practical Applications

• Operational productivity: true 24/7 capability with continuous loading and urgent sample prioritization, lowering labor costs and training requirements.
• Standardized workflow: fully automated preparation secures reproducible results and reduces infection risk via dedicated waste containers.
• Flexibility: compatible with Shimadzu and third-party assays or in-house laboratory developed tests; customizable LC–MS configuration with upgrade options.

Future Trends and Potential Applications

• Seamless integration with laboratory information systems for end-to-end automation of sample tracking, data acquisition and reporting.
• Wider adoption of high-resolution mass spectrometry (QTOF) for large-scale screening and untargeted metabolomics.
• Expansion into clinical diagnostics, therapeutic drug monitoring and pharmaceutical QA/QC requiring rapid multiplexed analyses.
• Development of advanced consumables and filter technologies to further reduce cross-contamination and increase throughput.

Conclusion

The CLAM-2040 automates and accelerates sample preparation for LC–MS in clinical research laboratories, delivering high throughput, reproducible results and improved safety. Its modular design and open configuration allow users to evolve methods, integrate new instrumentation and adapt to future analytical demands without compromise.