Agilent UV-Visible ChemStation for Spectroscopy Rev. B.05.01 - Specifications

Importance of UV-Visible Spectroscopy Software

UV-Visible spectroscopy is fundamental in chemical analysis, providing rapid and non-destructive measurements of sample absorbance across a wide wavelength range. Advanced software platforms integrate instrument control, data acquisition, and comprehensive evaluation, facilitating routine laboratory workflows, high-throughput screening, and compliance with regulatory standards.

Study Objectives and Overview

This document presents the specifications of Agilent ChemStation for UV-Visible Spectroscopy (Rev. B.05.01). It aims to describe the functionality, software modules, hardware compatibility, and system requirements of the platform, highlighting its modular design for general-purpose applications, biochemical analysis, dissolution testing, and electronic records compliance.

Methodology and Instrumentation

Key software modules:

• General Purpose (G1115AA)
• Advanced Analysis (G1116AA)
• Biochemical Analysis (G1117AA)
• Dissolution Testing (G1118AA)
• Security Pack (G1813AA)
• OpenLAB ECM Compliance Pack (G5182AA)

Instrument control and data acquisition are managed via GPIB or LAN interfaces for the Agilent 8453 diode-array spectrophotometer and Agilent 89090A Peltier temperature controller. Additional accessories include sipper/sampler systems and multicell transport. PC requirements feature a 1 GHz processor, 2 GB RAM, and Windows operating systems. Reporting supports ASCII and WMF output, with GLP-compatible workflows.

Key Results and Discussion

The software delivers multicomponent calibration, interactive spectral processing, time-resolved kinetics, thermal denaturation assays, and both offline and online dissolution testing for up to 24 vessels. Integration with OpenLAB ECM enables secure data storage, audit trails, electronic signatures, and user privilege management compliant with FDA 21 CFR Part 11. Customizable reporting and data reprocessing enhance flexibility and traceability in regulated environments.

Benefits and Practical Applications

• Streamlined routine and advanced spectroscopic analyses
• High-throughput biochemical kinetics and thermal melt assays
• Automated dissolution profiling with regulatory compliance
• Centralized user and data management via OpenLAB ECM
• Comprehensive reporting and audit trail generation

Future Trends and Potential Applications

Emerging directions include cloud-based data sharing, machine-learning algorithms for spectral deconvolution, expanded automation with robotics, and integration with laboratory information management systems (LIMS) to support digital transformation in analytical laboratories.

Conclusion

The Agilent UV-Visible ChemStation software provides a versatile, modular platform for comprehensive spectroscopic analysis across diverse applications. Its robust instrument control, advanced data processing, and compliance features meet the demands of research, quality control, and regulated environments.

References

• Agilent Technologies. Agilent ChemStation for UV-Visible Spectroscopy, Rev. B.05.01; Publication 5991-3448EN; October 17, 2013.