High-speed analysis of Artificial Dye

Význam tématu

Food additives often include synthetic colorants to enhance visual appeal. Rapid and reliable analysis of these dyes is essential for quality control and regulatory compliance. High-speed reversed-phase HPLC methods offer efficient separation and quantification of multiple dyes in a short analysis time.

Cíle a přehled studie

The study aims to develop a fast and robust chromatographic method for simultaneous detection of twelve common artificial food dyes at 50 mg/L each. Using Shimadzu Nexera XR and Shim-pack Scepter C18-120 column, the protocol targets a total runtime below five minutes.

Použitá metodika a instrumentace

• System Nexera XR
• Column Shim-pack Scepter C18-120, 100 mm L, 3.0 mm I.D., 1.9 μm
• Mobile phase A 10 mmol/L ammonium acetate, B 10 mmol/L ammonium acetate/acetonitrile 1/1 (v/v)
• Gradient 10% B to 100% B in 4 minutes, re-equilibration to initial conditions by 10 minutes
• Flow rate 0.9 mL/min, column temperature 40 °C, injection volume 5 μL
• Detection PDA at 254 nm and 630 nm

Hlavní výsledky a diskuse

Separation of all twelve dyes was achieved within 4 minutes with baseline resolution. Dual wavelength detection provided optimal sensitivity for different dye classes: aromatic dyes at 254 nm and azo/triarylmethane dyes at 630 nm. Peak shapes were sharp thanks to sub-2 μm particles, and method robustness was confirmed by reproducible retention times and peak areas across repeated injections.

Přínosy a praktické využití metody

Rapid screening of multiple synthetic colorants improves laboratory throughput and reduces solvent consumption. The method is well suited for routine quality control in the food industry and regulatory laboratories. Dual detection wavelengths enable direct confirmation of dye identity and accurate quantification without derivatization.

Budoucí trendy a možnosti využití

Integration with high-throughput autosamplers and multiplexed detection systems could further enhance productivity. Coupling HPLC with mass spectrometry may offer increased selectivity and structural confirmation. Advances in column technology and greener mobile phase formulations are expected to reduce environmental impact and operational costs.

Závěr

The developed reversed-phase HPLC method delivers fast, reliable analysis of a wide range of artificial food dyes in under five minutes. Its combination of high efficiency, reproducibility and dual-wavelength detection supports robust quality assurance workflows in food analysis.