Analysis of Chlorogenic acid in Vitamin C medicine

Significance of the Topic

Quantification of chlorogenic acid in pharmaceutical products ensures both efficacy and safety.
This polyphenolic compound contributes antioxidant properties and may influence the stability and performance of vitamin C formulations.

Objectives and Study Overview

This application note presents a reversed-phase liquid chromatography method for determining chlorogenic acid in vitamin C tablets.
Key goals include method development, sample preparation, and validation against a known standard.

Methodology and Instrumentation

• Column: Shim-pack VP-ODS (250 mm × 4.6 mm I.D., 5 µm)
• Mobile Phase: 1 % acetic acid aqueous solution : methanol = 94 : 6 (v/v)
• Flow Rate: 1.0 mL/min (isocratic)
• Column Temperature: 30 °C
• Injection Volume: 10 µL
• Detection: UV at 327 nm

Sample Pretreatment:

1. Collect and remove coating from 10 vitamin C tablets.
2. Grind tablets and weigh 1 g into a 100 mL volumetric flask.
3. Add methanol and perform ultrasonic extraction (300 W, 40 kHz) for 45 min.
4. Cool the solution, dilute to 100 mL with aqueous methanol, shake, and filter into an autosampler vial.

Main Results and Discussion

The standard chromatogram exhibited a well-defined chlorogenic acid peak at the expected retention time.
Analysis of the vitamin C sample confirmed chlorogenic acid present at levels comparable to the 30 µg/mL standard.
Excellent peak resolution and stable baselines supported precise quantitation.

Benefits and Practical Applications

• Provides reliable monitoring of polyphenol content in pharmaceutical products.
• Facilitates quality control in compliance with regulatory standards.
• Offers a straightforward workflow suited to routine laboratory testing.

Future Trends and Applications

Advances may include adoption of UHPLC for faster throughput, coupling with mass spectrometry for heightened selectivity, and extension of the method to additional polyphenolic compounds.

Conclusion

The described HPLC approach delivers a robust and reproducible protocol for measuring chlorogenic acid in vitamin C tablets, reinforcing product quality and consistency in pharmaceutical analysis.

References

Shimadzu Corporation. Analysis of Chlorogenic Acid in Vitamin C Medicine. ERAS-1000-0034, First Edition, March 2023.