FPO The Cary Eclipse—the only instrument for measurements using fiber optics

Significance of the Topic

Fluorescence spectroscopy is a fundamental technique in analytical chemistry for detecting and quantifying trace compounds based on their emission properties. Recent advances focus on improving flexibility, sensitivity, and ease of use to address complex samples and in situ measurements.

Objectives and Study Overview

This document introduces the Agilent Cary Eclipse fluorescence spectrophotometer, which uniquely integrates fiber optic remote sampling with proven immunity to ambient light. The aim is to extend fluorescence measurements to large or non-standard samples without compromising data quality.

Used Instrumentation

• Agilent Cary Eclipse fluorescence spectrophotometer
• Xenon flash lamp with no warm-up requirement and a 10-year lifetime guarantee
• Fiber optic probes for remote and in situ sampling
• Temperature control accessories for non-ambient conditions

Methodology

The Cary Eclipse employs a xenon flash excitation source coupled to sample probes via fiber optics. Integrated optical filters and robust detector electronics maintain signal integrity while eliminating interference from ambient light sources, enabling measurements with the sample compartment open.

Main Results and Discussion

Quinine solutions were measured with the sample compartment both open and closed. Emission spectra from 360 nm to 560 nm overlapped precisely, confirming complete roomlight immunity. This capability allows accurate fluorescence analysis in environments previously considered unsuitable.

Benefits and Practical Applications

• Lower operating costs and reduced downtime through long-life flash lamp technology
• Non-destructive, in situ measurements of large or immovable specimens
• Streamlined workflows with open-lid operation and easy sample access
• Enhanced laboratory safety by avoiding hazardous lamp changes
• Versatility across fluorescence, phosphorescence, chemiluminescence, and time-resolved assays

Future Trends and Potential Applications

• Integration with automated and remote sensing platforms for real-time monitoring
• Field deployment under extreme environmental conditions
• Advanced time-resolved and multiplexed fluorescence experiments
• Expanded use in environmental analysis, bioprocess monitoring, and industrial quality control

Conclusion

The Agilent Cary Eclipse sets a new benchmark in fluorescence spectroscopy by combining unmatched roomlight immunity with fiber optic sampling. It offers high sensitivity, operational flexibility, and reliable performance for a wide range of analytical applications.

References

1. Agilent Technologies, The Power of Xenon: Light Years Ahead in Fluorescence Measurement Technology, USA, March 11, 2016.