Agilent InfinityLab 96 Well Plate, 0.5 mL, Round Wells, U Shape, Polypropylene, 14 mm

Significance of the Topic

96-well plates are essential tools for high-throughput sample handling in analytical chemistry, particularly in chromatographic workflows. Their consistent geometry, chemical resistance, and compatibility with automated systems ensure reproducible sample preparation, storage, and analysis across diverse laboratories.

Objectives and Study Overview

This document reviews the design and performance attributes of the Agilent InfinityLab 96 Well Plate (0.5 mL round U-shape wells) intended for chromatography applications, sample collection, and long-term storage. It highlights structural features, material properties, and operational parameters to guide selection in QA/QC, research, and industrial settings.

Methodology and Instrumentation

• Plate material: Virgin medical-grade polypropylene with low biomolecular binding.
• Well design: 96 round, U-shaped wells each with 500 µL maximum and 450 µL working volume.
• Thermal range: Stable from –80 °C to 120 °C.
• Chemical compatibility: Resistant to DMSO, phenol, chloroform, and common lab reagents.
• Sealing: Flat clear surface compatible with silicone sealing mats (p/n 5042-1389).
• Format: SBS footprint, stackable, alphanumeric grid for tracking.

Key Results and Discussion

• Structural precision ensures uniform sealing and minimal cross-contamination.
• U-shaped well bottoms facilitate mixing efficiency and maximize sample recovery with autosamplers.
• Low thermal expansion and chemical inertness preserve sample integrity during incubation and reactions.
• Stackability and standardized footprint enable seamless integration with robotic handlers and plate readers.

Benefits and Practical Applications

• Enhanced throughput in LC, LC-MS, and automated dispensing platforms.
• Reliable storage of biological and chemical samples at varying temperatures.
• Reduced sample loss and adsorption, critical for low-abundance analytes.
• Efficient plate handling in clinical, environmental, and pharmaceutical laboratories.

Instrumentation Used

• Compatible with Agilent InfinityLab autosampler systems.
• Sealing mat: Silicone mat (p/n 5042-1389) for leak-proof operation.
• Defined for use in SBS-standard robotic plate handlers and incubators.

Future Trends and Potential Applications

Advancements in plate materials and surface coatings will further reduce adsorption of proteins and small molecules. Integration with smart lab automation and digital tracking will enhance data transparency. Custom well geometries and volumes will address emerging needs in microfluidics and single-cell analysis.

Conclusion

The Agilent InfinityLab 96 Well Plate offers a robust, versatile solution for chromatography and high-throughput sample management. Its material properties, well design, and compatibility with automated systems make it a reliable choice for diverse analytical workflows.

References

• Agilent Technologies. InfinityLab 96 Well Plate, 0.5 mL Round U-Shape, Polypropylene, 14 mm Data Sheet, 5994-6040EN, May 2023.