Analysis of Latamoxef Sodium (Shim-pack GIST C18-AQ)

Importance of the topic

Analysis of Latamoxef sodium is critical for ensuring the purity and potency of this oxacephem antibiotic widely used in clinical settings

Goals and overview

This application note presents a reversed-phase liquid chromatography method for separation and quantification of Latamoxef R and S enantiomers in aqueous samples using a Shim-pack GIST C18-AQ column

Methodology and instrumentation

A gradient elution protocol combines a phosphate buffer (pH 6.8) and acetonitrile to achieve baseline separation of the two enantiomers

• Column Shim-pack GIST C18-AQ (250 mm × 4.6 mm, 5 μm)
• Mobile phase A: 0.01 M dipotassium hydrogen phosphate buffer (pH 6.8); B: acetonitrile; gradient from 1 % to 60 % B over 47 min
• Flow rate 1.0 mL/min; column temperature 25 °C; injection volume 10 μL; detection at 225 nm
• Sample pretreatment: dilute 1 mL of Latamoxef sodium to 100 mL with water

Main results and discussion

The method demonstrates clear chromatographic resolution of Latamoxef R (retention ~9 min) and Latamoxef S (retention ~11 min) with a stable baseline over a 60-minute runtime

Benefits and practical applications

• Reliable enantiomeric discrimination supports quality control in pharmaceutical manufacturing
• Simple sample preparation streamlines routine analysis in QC laboratories
• UV detection at 225 nm provides sensitive response without derivatization

Future trends and potential applications

Advances in ultrahigh-performance columns and coupling with mass spectrometry may reduce analysis time and improve sensitivity for trace-level impurities in antibiotic formulations

Conclusion

The presented RP-LC method on a Shim-pack GIST C18-AQ column offers robust, reproducible separation of Latamoxef enantiomers, fulfilling the requirements for routine pharmaceutical analysis

Reference

Shimadzu Corporation ERAS-1000-0043 First Edition Mar. 2023