UV-VIS Spectrophotometer UV-2600i UV-2700i

Significance of the Topic

Ultraviolet–visible spectrophotometry is a cornerstone technique in analytical chemistry, enabling rapid, non-destructive measurement of molecular absorbance and reflectance. High-precision UV-Vis systems support quality control, environmental monitoring, materials research and regulatory compliance across industries such as electronics, pharmaceuticals, cosmetics, food and life sciences.

Study Objectives and Overview

This whitepaper introduces the Shimadzu UV-2600i single-monochromator and UV-2700i double-monochromator spectrophotometers. It reviews expanded wavelength ranges, ultra-low stray light performance, accessory options and software features that address diverse application needs—from band‐gap analysis of semiconductors to ultraviolet protection factor (UPF) testing of textiles.

Methodology and Instrumentation

The UV-2600i covers 185–900 nm (extendable to 1400 nm with the ISR-2600Plus integrating sphere) using a high-efficiency Lo-Ray-Ligh diffraction grating. The UV-2700i adds a second monochromator, delivering stray light down to 0.000001% (8 Abs). Key accessories include:

• ISR-2600/2600Plus integrating spheres for diffuse and specular reflectance
• MPC-2600A large-sample compartment for custom sample geometries
• Variable angle reflectance units and polarizers for thin films and multilayer coatings
• TMSPC-8i thermoelectric multicell holders for thermal melting (Tm) analysis of oligonucleotides
• ASX-560/280 and ASC-5 autosamplers for high-throughput liquid analysis

Data acquisition and compliance are managed via LabSolutions UV-Vis software, offering spectral evaluation, pass/fail judgments, real-time Excel® transfer, database management (LabSolutions DB/CS), and 21 CFR Part 11 support.

Main Results and Discussion

Performance benchmarks demonstrate:

• Clear band-gap determination of CuInGaSe₂ and CuInSe₂ via Tauc plots using an integrating sphere
• Thin film thickness calculations (polyvinylidene chloride, nylon, polypropylene) from interference waveforms with software automation
• Tm profiling of nucleic acid therapeutics over 9–60 µM ranges with thermoelectric cell holders
• Ultra-low stray light enabling absorbance linearity up to 8 Abs for potassium permanganate solutions
• Variable-angle reflectance shifts in multilayer dielectric coatings up to 70° incidence

These results confirm the instruments’ ability to handle high-concentration, low-transmittance and extended-wavelength analyses with exceptional reproducibility.

Benefits and Practical Applications

Shimadzu UV-2600i/2700i systems offer:

• Enhanced scalability into near-infrared regions (up to 1400 nm)
• Automated workflows via autosamplers and command-file control to reduce human error
• Comprehensive software modules for colorimetry, film thickness, UPF, solar transmittance and Tm analyses
• Rigorous data integrity through audit trails, version control and electronic signatures

This versatility supports environments from QA/QC labs in manufacturing to advanced research in materials science and life sciences.

Future Trends and Opportunities

Advances in digital connectivity (IoT/M2M) and artificial intelligence are poised to further automate instrument diagnostics, predictive maintenance and real-time data interpretation. Integration with cloud platforms and cross-instrument data management will enhance collaborative research and remote laboratory operations. Emerging applications include high-throughput screening of nanomaterials, real-time reaction monitoring in flow chemistry and smart factory deployments.

Conclusion

The Shimadzu UV-2600i and UV-2700i spectrophotometers combine cutting-edge optical design, modular accessories and robust software to address a broad spectrum of analytical challenges. Their ultra-low stray light, extended wavelength range and high-throughput capabilities deliver reliable, reproducible data for regulated and research environments alike.

References

No external literature citations were provided in the original document.