PurityChrom® & PurityChrom®MCC Plus

Significance of the Topic

High-performance liquid chromatography (HPLC) and preparative purification systems rely on automated data acquisition and precise instrument control. Modern chromatography software streamlines method design, real-time monitoring and post-run analysis, enhancing reproducibility, throughput and regulatory compliance in R&D, QC and industrial processes.

Objectives and Overview of the Manual

This instruction manual introduces PurityChrom® and its extended version PurityChrom® MCC Plus. Key aims are to guide users through installation, configuration and operation of the software’s modules for: instrument communication, method creation (time control and autosampler files), sequence automation, data recording, chromatogram reconstruction, peak detection, visualization of fluidics and waste/solvent management.

Methodology and Instrumentation

• Software environment: Windows-based, USB or LAN license dongle protection, USB/RS-232/Winsock device interfaces.
• Supported hardware: AZURA® pumps (P 2.1L, P 6.1L), UV detectors (UVD 2.1S/L, MWD), diode-array detectors (DAD 2.1L/6.1L), refractive index detector (RID 2.1L), conductivity/pH monitors (CM 2.1S), autosamplers (AS 6.1L variants), fraction collectors (standard valve and multiposition), event boxes, single-moving-bed (SMB) chromatography units, optional mass spectrometer 4000 MiD.
• Data acquisition: up to 8 (PurityChrom) or 16 (MCC Plus) analogue channels, customizable slice width, real-time gradient and threshold monitoring, automatic peak sampling.
• Method control: Graphical and tabular time control file editor, programmable functions (solvent composition, flow rate, valve switching, thresholds, annotations), peak windows, reference-peak alignment, method segments import/export, autosampler control files, sequence tables.
• User administration and audit trail: multi-level user roles, password policies, event logging for 21 CFR Part 11 compliance.

Main Features and Discussion

• Flexible method creation: graphical time table editor with chromatogram background, multi-unit programming (time/volume/column volume).
• Real-time control: on-screen pump and detector status, solvent supply and waste management displays, system fluidics visualization.
• Data handling: live chromatogram display, reconstruction of stored runs, superimposed or channel-by-channel comparison, reintegration tools with manual baseline/drop adjustment.
• Automation: fraction limiter, autosampler sequences, sample identifier table, programmable triggers via EventBox or game port, integration of special devices (heaters, flow meters, SMB).
• Reporting and compliance: automatic report generation, customizable printout templates, export to CSV/AIA formats, embedded audit trails.

Benefits and Practical Applications

PurityChrom® and MCC Plus enable end-to-end chromatography automation, reducing manual intervention and error. They support preparative fractionation, biomolecule purification, pharmaceutical QC, SMB operation and multi-pump gradient pumping. Modular licensing allows scalability to advanced multi-pump, multi-detector and single-moving-bed workflows.

Future Trends and Possibilities for Use

• Cloud integration for centralized method and data management across sites.
• AI-driven method optimization and real-time anomaly detection.
• Enhanced LIMS connectivity and regulatory automation.
• Expanded IoT sensor integration and remote diagnostics with mobile interfaces.
• Deepened MS coupling and advanced chemometric analysis.

Conclusion

PurityChrom® and PurityChrom® MCC Plus offer a comprehensive, modular platform for modern chromatographic control and data handling. Their blend of graphical programming, real-time monitoring and extensive device integration makes them well suited to research, industrial and quality environments seeking flexibility, automation and compliance.

References

None specified in the source document.