Bio-inert Multidraw Upgrade Kit G5667-68711

Importance of the Topic

The Bio-inert Multidraw Upgrade Kit expands the injection capabilities of Agilent 1260 Infinity Bio-inert Autosamplers and Multisamplers. By allowing extended injection volumes up to 1100 µL and maintaining a bio-inert flow path, this upgrade supports enhanced sensitivity for trace analysis of biomolecules and other sensitive compounds. The expanded delay volume and waste management considerations ensure reliable performance in biocompatible liquid chromatography workflows.

Objectives and Study Overview

This installation note details the mechanical and software configuration steps required to integrate the G5667-68711 Multidraw Upgrade Kit into two system variants: the Agilent 1260 Infinity Bio-inert Autosampler (G5667A) and the 1260 Infinity III Bio-inert Multisampler (G5668A). The goal is to guide users through capillary and fitting installations, zero dead-volume union setup, and controller parameter adjustments to activate multidraw mode.

Methodology and Instrumentation

Tools and parts required:

• Fitting mounting tool (p/n 5043-0915) or 5 mm hex wrench
• 1/4 inch wrench (autosampler accessory kit)
• Bio-inert ZDV union (p/n 5067-4741)
• PEEK sample loops of 2 mL (0101-1234) and 500 µL (0101-1236)

Key steps:

1. Install UHP-FF fittings onto capillaries, ensuring a 5 mm protrusion and torque of 0.8 Nm.
2. Assemble the zero dead-volume union in the correct sequence to avoid leaks.
3. Mount seat extension capillaries between the injection valve and existing seat capillary.
4. Connect dedicated waste tubing to prevent siphoning and protect injection precision.
5. Update controller settings in OpenLab CDS ChemStation, Lab Advisor, or Instant Pilot to reflect the extended capillary volume and enable multidraw mode.

Main Results and Discussion

Successful integration of the upgrade kit allows incremental sample loading into extended seat capillaries before valve switching. Performance considerations include:

• Delay volume doubles for extended capillaries; software bypass routines can minimize dead volume.
• PEEK sample loops exhibit solvent-dependent pressure limits (200–275 bar).
• Multidraw mode ejects displaced volume to waste, necessitating proper waste line setup.

Proper assembly and controller configuration ensure precise injections up to 1100 µL while maintaining bio-inert conditions.

Benefits and Practical Applications

The upgrade kit delivers:

• Enhanced sensitivity for low-abundance analytes through larger injection volumes.
• Compatibility with bio-inert flow paths to protect fragile biomolecules.
• Flexible volume programming for gradient or isocratic separations.

Ideal applications include biopharmaceutical QC, metabolomics, and trace-level environmental analysis where inert surfaces and high precision are critical.

Future Trends and Potential Applications

As demand grows for high-sensitivity and high-throughput bioanalysis, further enhancements may include automated capillary exchanges, dynamic volume scaling via software, and integration with online sample cleanup modules. Combining multidraw capability with microfluidic mixers or on-column concentration techniques could broaden application scope in proteomics and single-cell analysis.

Conclusion

The Bio-inert Multidraw Upgrade Kit (G5667-68711) provides a robust solution for increasing injection volumes in Agilent 1260 Infinity Bio-inert systems. Through clear mechanical assembly instructions and straightforward software configuration, laboratories can achieve precise, bio-inert sample introduction up to 1100 µL, enhancing analytical sensitivity without compromising system integrity.

Reference

Agilent Technologies. Bio-inert Multidraw Upgrade Kit G5667-68711 Installation Note. Part Number G5667-90123 Rev D. 2024.