Change the Project During a Running Sequence: Sequence Commands

Significance of the Topic

Effective management of high-throughput sample sequences is critical in polymer analysis and quality control. Dividing injections across multiple project folders during a run enhances data organization, reduces manual post-run processing, and supports multi-user workflows.

Objectives and Article Overview

This article demonstrates how to dynamically change project paths within an ongoing Agilent WinGPC sequence by inserting method change commands. It covers the setup, execution, and advantages of distributing injections to distinct data projects without interrupting the analysis.

Methodology and Instrumentation

• Software: Agilent WinGPC with Sequence Manager module.
• Control Interface: ChromPilot autosampler for automated vial handling.
• Procedure:
• Create baseline methods differing only in project path (e.g., PS and PMMA methods).
• In Sequence Manager, add “Load WinGPC method” actions at specified positions.
• Select the target method to switch the project folder mid-run.
• Ensure adequate injection interval to allow data acquisition restart.

Main Findings and Discussion

Dynamic method changes successfully routed the first three injections to one project and subsequent injections to another. The process operates seamlessly during overlapped injections when timing parameters are adjusted. Displaying the active method in the first sequence row aids real-time monitoring.

Benefits and Practical Applications

• Streamlines large‐batch polymer or multi‐user analyses by separating datasets automatically.
• Eliminates manual data segregation after a run, reducing human error.
• Allows assignment of different calibration curves or user identities within a single sequence.

Future Trends and Potential Applications

• Integration with centralized LIMS to automate project assignment based on sample metadata.
• Expanded support for dynamic method changes in other chromatographic or spectroscopic software.
• Enhanced scheduling features to optimize autosampler throughput.

Conclusion

Inserting WinGPC method change commands during an active sequence provides a flexible, efficient way to distribute injections across distinct project directories. This technique enhances workflow efficiency, data organization, and supports complex, multi-user analytical operations.

Reference

• Agilent Technologies. Change the Project During a Running Sequence: Sequence Commands. DE50548322, 5994-7470EN, January 21, 2025.