Analysis of Astaxanthin and Canthaxanthin

Analysis of Astaxanthin and Canthaxanthin Using Reversed-Phase LC on Shim-pack VP-ODS

Importance of the Topic
Carotenoids such as astaxanthin and canthaxanthin play a crucial role in food, feed and cosmetic industries due to their strong antioxidant activity and vibrant coloration. Precise quantification of these compounds is essential for product quality, regulatory compliance and research into their health-promoting properties.

Aims and Overview of the Study
This application note illustrates the development of a fast, robust reversed-phase liquid chromatography method to simultaneously separate and quantify astaxanthin and canthaxanthin using Shim-pack VP-ODS. It presents the analytical goals, experimental setup and assessment of chromatographic performance.

Methodology and Instrumentation
Chromatographic Conditions:

• Column: Shim-pack VP-ODS (150 mm × 4.6 mm I.D., 5 µm) with Shim-pack GVP-ODS guard column (10 mm × 4.6 mm I.D., 5 µm)
• Mobile Phase: 0.05 % trifluoroacetic acid / methanol (3/97, v/v)
• Flow Rate: 1.2 mL/min
• Column Temperature: 40 °C
• Injection Volume: 10 µL
• Detection: UV-Vis at 475 nm

Instrumentation

• Shimadzu HPLC system equipped with a UV-Vis detector and Shim-pack series columns

Key Results and Discussion
The optimized method achieved clear baseline separation of astaxanthin and canthaxanthin with sharp peak shapes and excellent sensitivity at 475 nm. Retention times were reproducible across replicate injections, confirming method precision.

Benefits and Practical Applications

• High Throughput: short analysis time supports routine quality control
• High Sensitivity: reliable detection of low concentrations
• Robustness: consistent performance over multiple runs
• Versatility: suitable for diverse matrices in food and cosmetic testing

Future Trends and Potential Applications
Advancements may include coupling with mass spectrometry for enhanced selectivity, miniaturized column formats for ultra-fast screening and automated sample preparation to improve laboratory efficiency. Growing interest in nutraceuticals could drive further method adaptation.

Conclusion
This reversed-phase LC approach on Shim-pack VP-ODS offers a straightforward, accurate and reproducible solution for quantitative analysis of astaxanthin and canthaxanthin, aiding research and quality assurance in various industries.