Clarity Control Module FC BY VICI VALCO VALVE

Significance of the Topic

The ability to automatically collect fractions in liquid chromatography ensures high sample purity and throughput, critical in pharmaceutical synthesis, biochemical analysis and quality control. Integration of the Vici Valco multiport valve into the DataApex Clarity environment delivers method-driven control, accurate timing and full documentation of sample collection events.

Objectives and Overview of the Manual

This manual provides a step-by-step guide to install, configure and operate the FC by Vici Valco Valve fraction collector within the Clarity chromatography station. It covers hardware requirements, communication setup, method creation and real-time monitoring features to streamline preparative workflows.

Methodology and Instrumentation Used

The control module communicates with the valve via RS-232 or USB using a Vici Valco-specific cable. Key components include:

• DataApex Clarity software with LC Control license.
• Free COM or USB port on the PC (use MultiCOM adapter if needed).
• UNI Ruby script (VALCOVALVEFC.RB) for device communication.
• DataApex UNI Setup dialog for port selection, baud rate and firmware version.

Main Features and Usage Workflow

Users first add the Vici Valco control module in System Configuration, assign it to the LC instrument and set digital I/O lines. Method setup comprises three tabs:

• Fraction Table: define flow rate, delay volumes, trigger conditions (level, slope or combined), filter settings and time windows for each fraction.
• Vial Numbers: specify starting and last vial positions, reset rules, waste or shutdown behavior and options such as ‘‘Collect All’’ or peak-top vial change.
• Properties: assign the waste port and enable vial recycling by resetting to the start position.

During acquisition, the Device Monitor window allows manual overrides (next vial, start/stop collection, go to position) and the Data Acquisition graph displays fraction events and delay markers.

Benefits and Practical Applications

• Automated, reproducible sample collection reduces manual labor and human error.
• Flexible trigger modes suit complex peak shapes and low-noise signals.
• Full method logging and real-time display enhance QA/QC and audit trails.
• Applicable in prep-HPLC, peptide purification, environmental sampling and routine QA labs.

Future Trends and Possibilities for Use

Emerging directions include integration with Internet-of-Things platforms for remote monitoring, AI-driven peak detection to optimize trigger settings, and expansion to microfluidic and high-throughput screening systems. Enhanced scripting interfaces and cloud-based data management will further streamline preparative chromatography workflows.

Conclusion

The FC by Vici Valco Valve control module in DataApex Clarity delivers a robust, user-friendly solution for automated fraction collection. Its comprehensive setup options, real-time monitoring and detailed reporting support high-precision separations and efficient laboratory operation.

References

DataApex Ltd. (2024). FC by Vici Valco Valve Control Module Manual (Code/Rev.: M163/90D). Prague, Czech Republic.