Electrolytes in Sports Beverages Using Acclaim Trinity P2

Význam tématu

Inorganic ions and quaternary ammonium compounds are key analytes in health and sports applications. This study addresses rapid and sensitive quantification of electrolytes and cetylpyridinium in commercial mouthwash and sports drinks. Accurate monitoring supports product quality and regulatory compliance.

Cíle a přehled studie

The objective was to develop a single chromatographic method for simultaneous separation and detection of mono and multivalent inorganic ions and cetylpyridinium. Sports beverages and mouthwash formulations were analyzed to verify label claims for sodium, potassium, magnesium, calcium and active antibacterial agents.

Použitá metodika a instrumentace

• Chromatographic system Thermo Scientific ICS 3000 ion chromatography and UltiMate 3000 RS liquid chromatography
• Columns Trinity P2 3×50 mm 3 µm and Acclaim Surfactant Plus 3×150 mm 3 µm
• Mobile phase gradient of water, acetonitrile, 100 mM formic acid and 100 mM ammonium formate at pH 3.65
• Flow rates of 0.5 and 0.6 mL/min; column temperatures 30 °C and 25 °C; injection volumes 5 and 2 µL
• Detectors: UV at 220 nm with blank subtraction and Corona Veo conductivity detection with suppressor current 8 mA
• Sample preparation using OnGuard II P cartridge for color removal

Hlavní výsledky a diskuse

The method achieved baseline separation of common electrolytes and cetylpyridinium. Retention times were reproducible, enabling clear peak identification for chloride, phosphate, citrate and quaternary ammonium species. Conductivity detection provided high sensitivity for inorganic ions while UV detection complemented cetylpyridinium quantification.

Přínosy a praktické využití metody

• Single run analysis reduces turnaround time for QA/QC laboratories
• High sensitivity and selectivity for diverse analytes ensures robust quality control
• Minimal sample preparation simplifies routine workflows

Budoucí trendy a možnosti využití

As demand grows for multifunctional beverages and health products, integrated chromatographic methods will be critical. Future work may include automation of sample cleanup, expansion to additional active ingredients and miniaturized systems for on‐site testing.

Závěr

The combined gradient approach on Trinity P2 and Acclaim Surfactant Plus columns with dual detection successfully quantifies electrolytes and cetylpyridinium in complex matrices. The protocol offers a robust solution for rapid quality assurance in pharmaceutical oral care and sports nutrition sectors.

Použitá instrumentace

• Thermo Scientific Dionex ICS 3000 ion chromatography system
• Thermo Scientific UltiMate 3000 RS liquid chromatography system
• Trinity P2 column 3×50 mm 3 µm
• Acclaim Surfactant Plus column 3×150 mm 3 µm
• Corona Veo conductivity detector with suppressor
• UV detector at 220 nm

Reference

Thermo Fisher Scientific application notes PB20627 E and PB20878 E