Orbitrap Excedion Pro hybrid mass spectrometer (Product specifications)

Importance of the Topic

The Orbitrap Excedion Pro hybrid mass spectrometer represents a major advancement in high-resolution mass spectrometry, combining high scan speeds, broad dynamic range and versatile fragmentation methods to address critical analytical challenges in biopharmaceutical characterization, proteomics, metabolomics and small molecule research.

Objectives and Overview

This summary reviews the Thermo Scientific Orbitrap Excedion Pro Hybrid Mass Spectrometer product specifications. It highlights the instrument’s design philosophy, performance metrics and integrated software ecosystem aimed at delivering enhanced sensitivity, accuracy and throughput for modern analytical workflows.

Methodology and Instrumentation

The system architecture integrates a high-field Orbitrap mass analyzer (4 kV central electrode) with an electrodynamic ion funnel (EDIF) and segmented quadrupole mass filter (AQT) to maximize ion transmission and mass accuracy. Fragmentation options include higher-energy collisional dissociation (HCD), electron transfer dissociation (ETD), combined ETD/HCD (EThcD) and hybrid data-independent acquisition (Hybrid-DIA). Adaptive retention time routines and Enhanced Dynamic Range (eDR) scan mode support both targeted and discovery experiments. Automatic Gain Control (AGC) and Advanced Peak Determination (APD) algorithms optimize ion injection and precursor annotation. Comprehensive software tools—Xcalibur, Ardia Platform, Chromeleon CDS, Proteome Discoverer, BioPharma Finder and Compound Discoverer—enable streamlined acquisition, processing and interpretation of complex datasets.

Instrumentation Used

• Thermo Scientific Orbitrap Excedion Pro Hybrid Mass Spectrometer
• OptaMax Plus and EASY-ETD ion sources
• High-capacity ion transfer tube and Electrodynamic Ion Funnel
• Orbitrap high-field mass analyzer
• Independent Charge Detector and Advanced Quadrupole Technology
• Thermo Scientific FAIMS Pro Duo interface (optional)

Key Results and Discussion

The Orbitrap Excedion Pro delivers up to 480,000 FWHM resolution at m/z 200 and scan rates up to 70 Hz. It supports a mass range of m/z 40–6,000 in standard mode and up to m/z 12,000 in the BioPharma Edition. Mass accuracy remains below 1 ppm using EASY-IC and internal lock mass calibration. The eDR mode achieves five orders of magnitude intra-scan dynamic range. Detection limits reach sub-femtogram levels for reserpine on column. TurboTMT enables accurate quantification of up to 32-plex TMT reagents, while Hybrid-DIA merges targeted PRM sensitivity with unbiased DIA discovery in a single run. The intuitive drag-and-drop method editor and preconfigured templates facilitate rapid method development and reproducible operation across large cohorts.

Benefits and Practical Applications

Enhanced sensitivity and selectivity support intact protein analysis, post-translational modification mapping, top-down proteomics and peptide mapping. Broad dynamic range enables quantitation of low-abundance biomarkers and metabolites. Versatile fragmentation modes yield complementary structural information for complex biomolecules. The modular software ecosystem ensures efficient data handling, automated reporting and compliance with regulated environments.

Future Trends and Potential Applications

Future developments may include tighter integration of ion mobility, AI-driven real-time acquisition strategies, expanded mass range for large biomolecules, and microflow or nanoflow interfaces optimized for single-cell proteomics. Cloud-based workflows and machine learning algorithms will further enhance throughput, data interpretation and predictive analytics, driving new discoveries in multi-omics research.

Conclusion

The Thermo Scientific Orbitrap Excedion Pro hybrid mass spectrometer sets a new standard in high-resolution, high-throughput mass analysis. Its combination of innovative hardware, versatile fragmentation capabilities and comprehensive software support addresses diverse analytical needs in biopharma, proteomics, metabolomics and small molecule investigations.

Reference

Thermo Fisher Scientific. Orbitrap Excedion Pro Hybrid Mass Spectrometer, Product Specifications PS-003701-EN, 2025.