Thermo Scientific VeriSpray PaperSpray ion source - Spot on: the faster path to MS analysis

Importance of the Topic

The VeriSpray PaperSpray ion source represents a significant advancement in mass spectrometry workflows by combining rapid, direct sampling with minimal sample preparation. This approach addresses the growing demand for high-throughput, cost-effective analytical methods in clinical research, environmental monitoring, food safety, and forensic toxicology. By eliminating extensive chromatographic steps, PaperSpray enhances laboratory productivity while reducing solvent usage and waste.

Objectives and Overview of the Article

This application note describes the design, functionality, and performance of the Thermo Scientific VeriSpray system. The primary goals are to:

• Introduce the hardware and software integration of the automated PaperSpray ion source.
• Detail operational parameters, sample throughput, and solvent consumption.
• Demonstrate compatibility with multiple Thermo Scientific mass spectrometers and robotic spotters.

Used Methodology and Instrumentation

The VeriSpray system streamlines direct ionization via consumable paper strips loaded into interchangeable plates. Key components include:

• VeriSpray PaperSpray ion source: automated recognition, adjustable X/Y/Z alignment, built-in barcode reader, and dual solvent reservoirs (Rewet and Spray Solvent dispensing 0–30 µL and 10–300 µL, respectively).
• Optional Plate Loader: removable ten-plate magazine handling up to 240 samples unattended.
• Thermo Scientific mass spectrometers: TSQ Altis, Quantis, Fortis, Quantiva, and Endura series.
• Software: Xcalibur 4.2 SP1 (or greater), TSQ Series II ICSW 3.2 for direct control, plus optional TraceFinder 4.1 SP5 for application-specific workflows.

Main Results and Discussion

Performance benchmarks demonstrate sample-to-sample analysis times of 1–2 minutes with negligible carryover. Automated acquisition can run continuously for an eight-hour shift, supported by large solvent reservoirs and low solvent consumption. The modular plate loader expands throughput without user intervention, enabling up to 240 analyses per cycle. Integration into instrument control software ensures seamless method execution and sample tracking via barcodes.

Benefits and Practical Applications

The VeriSpray system offers multiple advantages:

• Minimal sample preparation reduces turnaround time.
• Direct sampling avoids complex chromatography.
• Low solvent use and zero liquid waste lower operational costs and environmental impact.
• Automated plate handling and barcode tracking enhance reproducibility and data integrity.
• Scalable throughput meets demands of high-volume laboratories.

Future Trends and Potential Applications

Emerging directions for PaperSpray technology include further miniaturization of consumables, integration with machine-learning algorithms for real-time data interpretation, and expansion into new application areas such as therapeutic drug monitoring and environmental contaminant screening. Advances in robotics and workflow orchestration are expected to drive fully automated end-to-end analytical solutions.

Conclusion

The VeriSpray PaperSpray ion source simplifies mass spectrometry analysis by offering a rapid, high-throughput, and environmentally conscious workflow. Its compatibility with leading Thermo Scientific instruments and software platforms makes it an attractive solution for diverse analytical laboratories seeking efficient and reliable direct sampling methods.