Robust and Reproducible Protein Quantification in Plasma using the Evosep One and the Agilent 6495 Triple Quadrupole LC/MS | LabRulez LCMS
 

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Application NoteProteomicsPeptide Quantification in PlasmaUsing the Agilent 6495 TripleQuadrupole LC/MS Coupled with theAgilent 1290 Infinity II LC SystemAuthorLinfeng WuAgilent Technologies, Inc.AbstractThis application note showcases the quantitative performance of multiple reactionmonitoring (MRM)-based LC/MS analysis of peptides derived from proteinbiomarkers in human...
Key words
sis, sispeptide, peptidecounts, countspeptides, peptidesplasma, plasmaacquisition, acquisitiontime, timequantitative, quantitativesgipivtspyqihftk, sgipivtspyqihftkmin, minmrm, mrmquantification, quantificationlloq, lloqhuman, humanaflltpr
The Agilent 6495 Triple QuadrupoleLC/MS: Peptide QuantitationPerformanceTechnical OverviewIntroductionSample complexity and the low concentration of certain biomarkers are the majorchallenges encountered when performing biomarker verification using multiple reactionmonitoring (MRM)-based methods. For this reason, the development of MRM-basedanalytical methods using stable isotope-labeled...
Key words
contest, contestnanoflow, nanoflowpeptide, peptideflow, flowplasma, plasmaquantitation, quantitationcounts, countsfmol, fmolquantitative, quantitativestd, stdperformance, performancestandard, standardpeptides, peptidesamol, amolhed
Poster ReprintASMS 2020ThP487Robust and Reproducible ProteinQuantification in Plasma usingEvosep One and Agilent 6495Triple Quadrupole LC/MSLinfeng Wu 1 and Nicolai Bache 21AgilentTechnologies, Santa Clara, CA;2EvosepBiosystems, Odense, Denmark IntroductionExperimentalIn clinical research applications, it is commonlynecessary to analyze low abundance proteinbiomarkers from large sample...
Key words
amol, amolpeptide, peptidesis, sisrobustness, robustnessplasma, plasmananospray, nanospraypeptides, peptidesevotips, evotipscolumn, columnevosep, evoseprsd, rsdresponses, responsesprotein, proteinreproducibility, reproducibilitytest
Poster ReprintASMS 2022Poster number WP059An Easier, More Robust, and Faster Lowflow Quantitative Proteomics ApproachWendi A. Hale1, Laurent Rieux2, Nicolai Bache2, ClaudiaGaither3, René P. Zahedi4,5, Christoph H. Borchers4,5,6Agilent, Lexington, MA, United States2 Evosep, Odense, Denmark3MRM Proteomics Inc., Montréal, QC, Canada4 Segal...
Key words
evosep, evosepdifference, differenceskyline, skylinepeptides, peptidessensitivity, sensitivitymrm, mrmthroughput, throughputpeptiquant, peptiquantproteomics, proteomicsrehydrated, rehydratedquantitation, quantitationinject, injectisotope, isotopeanalytical, analyticallabeled
 

Similar PDF

Toggle
Application NoteProteomicsPeptide Quantification in PlasmaUsing the Agilent 6495 TripleQuadrupole LC/MS Coupled with theAgilent 1290 Infinity II LC SystemAuthorLinfeng WuAgilent Technologies, Inc.AbstractThis application note showcases the quantitative performance of multiple reactionmonitoring (MRM)-based LC/MS analysis of peptides derived from proteinbiomarkers in human...
Key words
sis, sispeptide, peptidecounts, countspeptides, peptidesplasma, plasmaacquisition, acquisitiontime, timequantitative, quantitativesgipivtspyqihftk, sgipivtspyqihftkmin, minmrm, mrmquantification, quantificationlloq, lloqhuman, humanaflltpr
The Agilent 6495 Triple QuadrupoleLC/MS: Peptide QuantitationPerformanceTechnical OverviewIntroductionSample complexity and the low concentration of certain biomarkers are the majorchallenges encountered when performing biomarker verification using multiple reactionmonitoring (MRM)-based methods. For this reason, the development of MRM-basedanalytical methods using stable isotope-labeled...
Key words
contest, contestnanoflow, nanoflowpeptide, peptideflow, flowplasma, plasmaquantitation, quantitationcounts, countsfmol, fmolquantitative, quantitativestd, stdperformance, performancestandard, standardpeptides, peptidesamol, amolhed
Poster ReprintASMS 2020ThP487Robust and Reproducible ProteinQuantification in Plasma usingEvosep One and Agilent 6495Triple Quadrupole LC/MSLinfeng Wu 1 and Nicolai Bache 21AgilentTechnologies, Santa Clara, CA;2EvosepBiosystems, Odense, Denmark IntroductionExperimentalIn clinical research applications, it is commonlynecessary to analyze low abundance proteinbiomarkers from large sample...
Key words
amol, amolpeptide, peptidesis, sisrobustness, robustnessplasma, plasmananospray, nanospraypeptides, peptidesevotips, evotipscolumn, columnevosep, evoseprsd, rsdresponses, responsesprotein, proteinreproducibility, reproducibilitytest
Poster ReprintASMS 2022Poster number WP059An Easier, More Robust, and Faster Lowflow Quantitative Proteomics ApproachWendi A. Hale1, Laurent Rieux2, Nicolai Bache2, ClaudiaGaither3, René P. Zahedi4,5, Christoph H. Borchers4,5,6Agilent, Lexington, MA, United States2 Evosep, Odense, Denmark3MRM Proteomics Inc., Montréal, QC, Canada4 Segal...
Key words
evosep, evosepdifference, differenceskyline, skylinepeptides, peptidessensitivity, sensitivitymrm, mrmthroughput, throughputpeptiquant, peptiquantproteomics, proteomicsrehydrated, rehydratedquantitation, quantitationinject, injectisotope, isotopeanalytical, analyticallabeled
 

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