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Application NoteProteomicsPeptide Quantification in PlasmaUsing the Agilent 6495 TripleQuadrupole LC/MS Coupled with theAgilent 1290 Infinity II LC SystemAuthorLinfeng WuAgilent Technologies, Inc.AbstractThis application note showcases the quantitative performance of multiple reactionmonitoring (MRM)-based LC/MS analysis of peptides derived from proteinbiomarkers in human...
Key words
sis, sispeptide, peptidecounts, countspeptides, peptidesplasma, plasmaacquisition, acquisitiontime, timequantitative, quantitativemin, minsgipivtspyqihftk, sgipivtspyqihftkquantification, quantificationmrm, mrmlloq, lloqhuman, humanaflltpr
The Agilent 6495 Triple QuadrupoleLC/MS: Peptide QuantitationPerformanceTechnical OverviewIntroductionSample complexity and the low concentration of certain biomarkers are the majorchallenges encountered when performing biomarker verification using multiple reactionmonitoring (MRM)-based methods. For this reason, the development of MRM-basedanalytical methods using stable isotope-labeled...
Key words
contest, contestnanoflow, nanoflowpeptide, peptideflow, flowplasma, plasmaquantitation, quantitationcounts, countsfmol, fmolquantitative, quantitativeperformance, performancepeptides, peptidesstd, stdprotein, proteinstandard, standardaccuracy
Poster ReprintASMS 2020ThP487Robust and Reproducible ProteinQuantification in Plasma usingEvosep One and Agilent 6495Triple Quadrupole LC/MSLinfeng Wu 1 and Nicolai Bache 21AgilentTechnologies, Santa Clara, CA;2EvosepBiosystems, Odense, Denmark IntroductionExperimentalIn clinical research applications, it is commonlynecessary to analyze low abundance proteinbiomarkers from large sample...
Key words
peptide, peptideamol, amolsis, sisrobustness, robustnessplasma, plasmapeptides, peptidesnanospray, nanosprayevotips, evotipscolumn, columnevosep, evoseprsd, rsdprotein, proteinreproducibility, reproducibilityresponses, responsestest
Agilent LC Triple QuadrupoleClinical Research CompendiumApplication Compendium Table of contentsContentsIntroduction4Routine analysis5Hormones5Progesterone Metabolism in Serum5Analytical Determination of Testosterone in Human Serum Using anAgilent Ultivo Triple Quadrupole LC/MS7Plasma Catecholamines by LC/MS/MS Using Agilent Captiva NDLipids,BondElut PBA SPE, 1290 Infinity LC, and 6460 Triple...
Key words
atturos, atturosplasma, plasmacounts, countsstreamselect, streamselectagilent, agilentserum, serummel, meltriple, triplecaptiva, captivaquadrupole, quadrupoleusing, usingthc, thcmethod, methodtestosterone, testosteroneacquisition
 

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Application NoteProteomicsPeptide Quantification in PlasmaUsing the Agilent 6495 TripleQuadrupole LC/MS Coupled with theAgilent 1290 Infinity II LC SystemAuthorLinfeng WuAgilent Technologies, Inc.AbstractThis application note showcases the quantitative performance of multiple reactionmonitoring (MRM)-based LC/MS analysis of peptides derived from proteinbiomarkers in human...
Key words
sis, sispeptide, peptidecounts, countspeptides, peptidesplasma, plasmaacquisition, acquisitiontime, timequantitative, quantitativemin, minsgipivtspyqihftk, sgipivtspyqihftkquantification, quantificationmrm, mrmlloq, lloqhuman, humanaflltpr
The Agilent 6495 Triple QuadrupoleLC/MS: Peptide QuantitationPerformanceTechnical OverviewIntroductionSample complexity and the low concentration of certain biomarkers are the majorchallenges encountered when performing biomarker verification using multiple reactionmonitoring (MRM)-based methods. For this reason, the development of MRM-basedanalytical methods using stable isotope-labeled...
Key words
contest, contestnanoflow, nanoflowpeptide, peptideflow, flowplasma, plasmaquantitation, quantitationcounts, countsfmol, fmolquantitative, quantitativeperformance, performancepeptides, peptidesstd, stdprotein, proteinstandard, standardaccuracy
Poster ReprintASMS 2020ThP487Robust and Reproducible ProteinQuantification in Plasma usingEvosep One and Agilent 6495Triple Quadrupole LC/MSLinfeng Wu 1 and Nicolai Bache 21AgilentTechnologies, Santa Clara, CA;2EvosepBiosystems, Odense, Denmark IntroductionExperimentalIn clinical research applications, it is commonlynecessary to analyze low abundance proteinbiomarkers from large sample...
Key words
peptide, peptideamol, amolsis, sisrobustness, robustnessplasma, plasmapeptides, peptidesnanospray, nanosprayevotips, evotipscolumn, columnevosep, evoseprsd, rsdprotein, proteinreproducibility, reproducibilityresponses, responsestest
Agilent LC Triple QuadrupoleClinical Research CompendiumApplication Compendium Table of contentsContentsIntroduction4Routine analysis5Hormones5Progesterone Metabolism in Serum5Analytical Determination of Testosterone in Human Serum Using anAgilent Ultivo Triple Quadrupole LC/MS7Plasma Catecholamines by LC/MS/MS Using Agilent Captiva NDLipids,BondElut PBA SPE, 1290 Infinity LC, and 6460 Triple...
Key words
atturos, atturosplasma, plasmacounts, countsstreamselect, streamselectagilent, agilentserum, serummel, meltriple, triplecaptiva, captivaquadrupole, quadrupoleusing, usingthc, thcmethod, methodtestosterone, testosteroneacquisition
 

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