Multi-Attribute Analysis of Monoclonal Antibodies Using the Agilent InfinityLab 2D-LC Solution and Q-TOF MSApplications | 2020 | Agilent TechnologiesInstrumentationLC/TOF, LC/HRMS, LC/MS, LC/MS/MS, 2D-LCIndustriesPharma & BiopharmaManufacturerAgilent TechnologiesKey wordsmab, clone, protein, mau, sec, heart, cutting, tocilizumab, actemra, counts, originator, supernatants, variants, main, titer, dimension, cho, clones, lmw, min, mol, column, cell, mobile, from, valve, therapeutic, deconvoluted, pump, rplc, attribute, multiple, glycosylation, chromatography, gradient, affinity, loop, phase, culture, producing, area, size, detection, amu, mass, antibodies, monoclonal, desalted, timetable, diverter
Similar PDF
2D-LC/MS Characterization of Charge Variants Using Ion Exchange and Reversed-Phase Chromatography
2020|Agilent Technologies|Applications
2D-LC/MS Characterization of ChargeVariants Using Ion Exchange andReversed-Phase ChromatographyMultiple Heart-Cutting 2D-LC Analysis of Innovatorversus Biosimilar Monoclonal AntibodiesApplication NoteBiotherapeutics and BiologicsAuthorAbstractSonja SchneiderThe determination of charge variants is one of the most important qualityAgilent Technologies, Inc.attributes for the characterization of biopharmaceuticals such...
Key words
dimension, dimensionbiosimilar, biosimilarsalt, saltinnovator, innovatorvariants, variantscharge, chargerituximab, rituximabfirst, firstwaste, wastemain, mainsecond, secondbuffer, bufferphosphate, phosphatevariant, variantgradient
Agilent AdvanceBio Desalting-RP Cartridges for Online Desalting in 2D‑LC/MS mAb Analysis
2016|Agilent Technologies|Applications
Agilent AdvanceBio Desalting-RPCartridges for Online Desalting in2D‑LC/MS mAb AnalysisApplication NoteBiotherapeutics and BiologicsAuthorsAbstractSuresh Babu C.V. and Ravindra GudihalThis Application Note describes the use of a desalting cartridge in the secondAgilent Technologies, Inc.dimension to desalt and characterize monoclonal antibodies (mAbs) usinga 2D-LC/MS...
Key words
desalting, desaltingadvancebio, advancebiodimension, dimensionmab, mabsecond, seconddeconvoluted, deconvolutedinnovator, innovatoragilent, agilentbiosimilar, biosimilarprotein, proteinheart, heartcutting, cuttingmau, maucartridges, cartridgesvariants
Clone Selection Using the Agilent 1290 Infinity Online 2D-LC/MS Solution
2016|Agilent Technologies|Applications
Clone Selection Using theAgilent 1290 Infinity Online 2D-LC/MSSolutionApplication NoteBiopharmaceuticalsAuthorsAbstractRavindra Gudihal andThis Application Note describes the Agilent 1290 Infinity online 2D-LC/MSSyed Salman Lateefsolution and proof of principle for clone selection and quantification of monoclonalAgilent Technologies, India Pvt Ltd,antibody (mAb) using an...
Key words
counts, countsbiosimilar, biosimilarclone, clonedeconvoluted, deconvolutedamu, amuinnovator, innovatormass, masscharge, chargepurification, purificationagilent, agilentdesalting, desaltingrituximab, rituximabprotein, proteinmab, mabmin
Application NoteBiopharma/PharmaDetermination of Multiple Attributesof Monoclonal AntibodiesSimultaneous and parallel multi-attribute analysisusing 3D-LC/MS with 2D multimethod optionmAbSECSize variantsProtein ACEXLC/MSmAb TiterCharge variantsMol wt (MW)AA sequencePTMCell culturesupernatantsHICHydrophobicvariantsHost cell materialAuthorsLiesa Verscheure,Gerd Vanhoenacker,Pat Sandra, and Koen SandraRIC biologicsPresident Kennedypark 26B-8500 KortrijkBelgiumSonja Schipperges,Sonja Schneider, andUdo HuberAgilent...
Key words
trastuzumab, trastuzumaboriginator, originatorcex, cexmau, mauhic, hiccho, chohmw, hmwprotein, proteinvariants, variantscounts, countsdeconvoluted, deconvolutedclones, clonesclone, clonevalve, valvemin