Thermo Scientific Vanquish HPLC and UHPLC Systems

Vanquish HPLC and  
UHPLC Systems

LC that takes  

your productivity  

to new heights

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Today’s scientists need to solve the challenges faced to produce life-saving medicines 
and improve the quality of our food and the environment. This work deserves the 
best analytical tools available; tools that empower you to achieve your next scientific 
breakthrough, measure the previously unmeasurable, and deliver outstanding quality 
results dependably. 

Designed with innovative technology and attention to fine detail, Thermo Scientific™ 
Vanquish™ HPLC and UHPLC systems are the most advanced LC instruments 
available. The Vanquish systems improve performance and repeatability with no  
trade-offs in quality, robustness, or ease-of-use. Regardless of which Vanquish HPLC 
or UHPLC system is used, chromatographers have all they need to solve their 
toughest analytical challenges with confidence.

• Deliver results without compromise—Unsurpassed retention time 

and peak area precision 

• Easier method development and routine analysis—Dedicated tools 

for fast method development and validation with exceptional instrument 
robustness to maximize uptime 

• Improve productivity—Better throughput, improved sample 

characterization, and faster return on investment 

• Detect the lowest sample quantities—Higher detector sensitivity  

and lower baseline noise 

• Seamlessly integrate MS—Dedicated kits and software solutions 

provide exceptional LC-MS performance 

• Maximize your results—Easy to use, control, and process data with 

Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)

Thermo Scientific™ Vanquish™ 
Core HPLC system
Absolute dependability to enable 
worry-free applications

No trade-offs in performance, robustness or ease-of-use  

Get more from liquid chromatography

Thermo Scientific™ Vanquish™ 
Horizon UHPLC system
Unrivaled performance and 
throughput for applications 
requiring high-end UHPLC

For any application—whether HPLC or UHPLC, small or 
large molecules, simple or complex mixtures—the Vanquish 
platform was designed to handle it all. This innovative, 
simple-to-operate, and easy-to-maintain platform delivers 
confident separations that push chromatographic boundaries. 
Regardless of the configuration you choose, you will get a 
highly integrated solution with optimized fluidic connections, 
providing unsurpassed retention time and peak area precision.

Thermo Scientific™ Vanquish™ 
Flex UHPLC systems
Complete flexibility for method 
development or fast and reliable 
UHPLC

Thermo Scientific™ Vanquish™ 
Duo HPLC and UHPLC systems
Increased productivity with two 
completely independent flow paths 
for higher throughput and improved 
sample characterization

Run more samples, unattended

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Vanquish Horizon UHPLC system
The Thermo Scientific Vanquish Horizon UHPLC system is an 
integrated, fully biocompatible state-of-the-art binary UHPLC system 
with ultra-low gradient delay volume, designed to provide unrivaled 
performance for high resolution and high-throughput LC and LC-MS 
applications. 

Why SmartInject Technology? 
Unlike traditional UHPLC systems, Vanquish 
LC systems utilize SmartInject Technology.  
A novel injection feature that significantly 
reduces dramatic pressure pulses that would 
otherwise lead to reduced column lifetime  
and challenges with retention time precision. 
SmartInject Technology enables improved 
data confidence and reduced cost of 
ownership. 

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Ultra high peak capacity for a complex herbal extract analysis.

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Peak capacity of 720 at 1360 bar

 Retention time [min]

Absorbance [mAU]

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Impurity profiling of nevirapine (0.66 mg/mL) with UHPLC  
gradient and zoom into the baseline to show related impurities.

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mAU

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Minutes

-500

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Nevirapine

Minutes

3500

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Significant reduction of pressure drop after injection using SmartInject 
Technology resulting in improved retention time reproducibility (6 replicates) 
and enhanced column lifetime.

System Pressure Trace

Time [min]

Inject

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Without SmartInject

No pressure drop with SmartInject

Pressure drop

Pressure [bar]

UV Trace with SmartInject

Absorbance [mAU]

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Best  precision

Deliver results without compromise

Vanquish Flex UHPLC systems
The Thermo Scientific Vanquish Flex systems offer binary and 
quaternary solvent blending. Binary solvent blending results in low 
gradient delay volumes that are ideal for routine UHPLC and LC-MS 
applications. Quaternary solvent blending allows multicomponent 
gradient formation for flexible UHPLC method development. 

Vanquish Core HPLC system
The Thermo Scientific Vanquish Core HPLC systems offers highly dependable  
HPLC analysis for binary, quaternary and isocratic solvent blending. A new level of 
user experience, the highest system productive time, and its ease to adopt within  
any given laboratory infrastructure make it ideal for all traditional HPLC analyses. 

High-throughput pesticides 
analysis by full scan LC-MS 
presented as extracted ion 
chromatogram.

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Retention Time [min]

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Rel. Abundance [%]

275

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285

m/z

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m/z

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=  System Suitability Test (SST) passed

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Test sites of a Vanquish Core system round robin test using eight different instruments with individual 
samples, users, and columns. Each analysis passed the specified SST criteria highlighting the reliability 
of the instrument and the ease of use for each user resulting in a successful SST.

Easier method development and routine analysis

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Vanquish Duo for Dual LC
Double your throughput deepen your 
sample knowledge and better utilize 
your bench space with the Vanquish 
Duo for Dual LC workflow. Two 
completely independent flow paths for 
maximum productivity.

Vanquish Duo for  
Tandem LC or LC-MS
Increase your lab’s throughput by 30%  
with the Vanquish Duo for Tandem LC  
or LC-MS workflow.1 Maximize the 
utilization of your optical detector or 
mass spectrometer.

Vanquish Duo UHPLC systems 

The Thermo Scientific Vanquish Duo UHPLC systems improve 
productivity and sample knowledge to deliver a faster return on 
investment. The Vanquish Duo UHPLC systems expand the 
benefits of our award-winning Vanquish platform with four 
workflows (Dual LC, Tandem LC or LC-MS, Transcend Duo LX-2, 
and Inverse Gradient). Vanquish Duo UHPLC systems use a 
separate second flow path in one integrated system set up by 
using Vanquish Flex or Vanquish Horizon modules.

Four workflows. Two flow paths.  

One integrated UHPLC solution.

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Vanquish Duo for  
Transcend Duo LX-2
Double the throughput of your LC-MS 
application with the Vanquish Duo for 
Transcend Duo LX-2. Run up to two 
applications simultaneously with a  
multi-channel LC while benefiting from  
the robustness, performance, and ease of 
use inherent of the Vanquish LC platform.

Vanquish Duo for  
Inverse Gradient LC
Improve your lab’s quantification 
capabilities with the Vanquish Duo for 
Inverse Gradient, employing the unique 
and universal Charged Aerosol Detection, 
to quantify all non-volatile and semi-volatile 
compounds even when no individual 
reference standard is available.

Flow Path 1

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Application

Switch Time 

Analytical Gradient

Application 2

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Analytical Gradient

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Analytical Gradient

Application 2

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Analytical Gradient

Application 2

Application

Switch Time

Single Channel LC

Flow Path 1

Flow Path 2

Application

Switch Time

Analytical Gradient

Application 2

Analytical Gradient

Application 2

Time savings

Compared to single channel LC

Tandem LC or LC-MS

Time savings

Compared to single channel LC

Flow Path 2

Dual LC

Analytical Gradient

Application 1

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Analytical Gradient

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Analytical Gradient

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Flow Path 1

Flow Path 2

Dual LC

Analytical Gradient

Application 1

Analytical Gradient

Application 1

Flow Path 1

Flow Path 2

Resulting

MS Signal

Multichannel LC-MS

Analytical Gradient

Application 1

Analytical Gradient

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Analytical Gradient

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Analytical Gradient

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Flow Path 1
Flow Path 2

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Inverse Gradient Method — Improved quantification with CAD

Analytical Gradient

Application 1

Analytical Gradient

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Analytical Gradient

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Powerful fluorescence detection
Fluorescence detection enables high sensitivity detection of 
fluorescent compounds with a long-life lamp technology.

Cost-effective, reliable UV-Vis
UV-Vis detection with excellent 
sensitivity and linearity is available 
to streamline in targeted compound 
analysis.

Choosing the right detection technology is key to revealing all the 
components of interest in your sample. The Vanquish HPLC and 
UHPLC platforms offers a wide range of detection capabilities that 
can be easily integrated and combined to fit your methods. 

FLD chromatogram of the four aflatoxins: G2, G1, B2 and B1 at a 
concentration of 0.9 µg/kg for G2 and B2 and 2.9 µg/kg for G1 and B1.

Industry-leading diode array detection
The industry-leading Thermo Scientific™ Vanquish™ 
diode array detectors offers the widest linear range and 
excellent signal-to noise performance, exceeding that 
of variable wavelength detectors. For extremely 
demanding applications, increase your detection 
sensitivity with the Thermo Scientific™ Diode Array 
Detector HL using Thermo Scientific™ LightPipe™ flow 
cells. LightPipe technology achieves excellent signal-
to-noise performance with low baseline noise and 
drift, and low peak dispersion. Leverage the Thermo 
Scientific™ Diode Array Detector FG or CG for the 
highest linear range and smooth LC-MS coupling. 

Concentration vs. peak height plot of nevirapine with data 
points that were considered for calibration (black) and data 
points that were eliminated from calibration due to curve 
decline (red). Linear calibration with permitted offset and 
no weighting.

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Peak Height [mAU]

Nevirapine concentration [µg/mL]

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Retention time [min]

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oun

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G2

G1

B2

B1

Detect the lowest sample quantities

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Uniquely universal charged aerosol detection
The Thermo Scientific™ Vanquish™ charged aerosol 
detector is powerful and universal, able to detect 
virtually any large and small molecule that lacks  
a chromophore, or that poorly ionizes, with  
sub-nanogram-level sensitivity and near-uniform 
response. The flexibility and performance of charged 
aerosol detection is ideal for analytical R&D, while its 
simplicity and reproducibility benefit manufacturing 
QA/QC applications.

Single quadrupole mass detection
Mass spectrometry gives access to valuable data no 
other technology can deliver. The easy to use Thermo 
Scientific™ ISQ™ EM single quadrupole mass 
spectrometer or the Thermo Scientific™ ISQ™ EC 
single quadrupole mass spectrometer integrates with 
LC systems for reliable, robust, and easy LC-MS 
routine analysis with an extended mass range for 
more flexibility. Integrated software allows both 
novices and experts to quickly master MS to gain 
more insights from every sample.

Unlike mass spectrometry (A) and UV (B), the CAD is able to  
measure all analytes in the sample. Mass spectrometry (MS)  
requires the analyte to form gas phase ions while response  
by a UV detector depends upon the nature of the chromophore.

0 

2 

4

Time [min]

Retention time [min]

LC-MS TIC positive ionscan m/z 250−1250 

Erucamide

Stearic acid

Palmitic

acid

Tinuvin

234

UV at 220 nm

Charged Aerosol Detection

0 

0.5 

1 

1.5  

2

Void + Cl-

Propanolol

Ketoprofen

Verapamil

UV at 220 nm

UV at 254 nm

Charged 
Aerosol 
Detection

The Vanquish platform combines seamlessly with Thermo 
Scientific™ mass spectrometers, providing an extra level of 
information, confidence and productivity. Mass spectrometry 
provides sensitivity and selectivity for your analyses, giving you 
more insight into your samples and ability to resolve difficult 
separations, including co-eluting peaks, using differing  
mass-to-charge (m/z) ratios.

Get better LC connections
Thermo Scientific™ Viper™ fingertight fittings revolutionize the way 
UHPLC connections are made. Viper fittings pave the way for 
easy setup of virtually dead-volume-free chromatography, even 
for your most advanced column configurations. Now you can 
enjoy tool-free system fluidics setup and other connections.

Viper fingertight fittings

Thermo Scientific Vanquish system with the Thermo Scientific™ Orbitrap
Exploris™ 480 mass spectrometer. 

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Seamlessly integrate MS

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One CDS to do it all
Benefit from the industry-leading, easy-to-use and compliant Chromeleon 
CDS with eWorkflow™ procedures for error-free sequence and method setup, 
dedicated ePanels for direct instrument control, walk-up Open Access 
software, and smart tools to streamline data processing and reporting. 
Chromeleon CDS delivers advanced system communication with single-point 
intelligent control and functionality, and provides plugins for the Vanquish 
UHPLC systems.

Optimize your separations with Thermo Scientific columns
The Vanquish HPLC and UHPLC systems are designed around the 
column, the core to every LC separation. Our family of Thermo 
Scientific™ Vanquish™ LC columns, partnered with the Vanquish 
platform to take advantage of its extended pressure capabilities 
and robustness allowing you to optimize your separations faster and 
more easily. In addition, all Vanquish LC valves are biocompatible, 
have a long-lifespan, and low maintenance, meeting the 
performance needs to make LC run robustly and routinely. 

Chromeleon CDS for unparalleled Vanquish 
UHPLC system control.

Maximize your results

Find out more at  
thermofisher.com/liquidchromatography

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UNITY LAB SERVICES

Laboratories are constantly asked to do more with less. Built from the ground up,  
Thermo Scientific HPLC and UHPLC instruments enable you to raise your productivity 
to the next level and give you confidence in your results. Time and cost associated 
with staff training are minimized allowing your laboratory to meet ever-increasing 
productivity demands, making it faster to bring products to market. With the largest 
portfolio of LC solutions, we remain a steadfast and committed partner in your 
endeavor to improve the world around us.

LC that takes your productivity to new heights 

The collective power  

of chromatography

References
1.  Thermo Fisher Scientific. TN72203: Tandem UHPLC operation for high-throughput LC-MS peptide 

mapping analyses, 2018, Germering, Germany. https://assets.thermofisher.com/TFS-Assets/
CMD/Technical-Notes/tn-72203-tandem-uhplc-peptide-mapping-tn72203-en.pdf