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Pressurized Online Pepsin Digestion of mAb IgG2 forHydrogen Deuterium Exchange Mass SpectrometryJing Fang, Ying Qing Yu, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITSINTRODUCTIONAn enhanced-pressure (up to ~ 15,000 psi),Pepsin digestion is an integral part of hydrogen deuterium exchange masssustainable...
Key words
digestion, digestionpepsin, pepsinchain, chainhdx, hdxquench, quenchdeuterium, deuteriumcoverage, coveragepeptides, peptidesheavy, heavybradykinin, bradykininsequence, sequencelight, lightredundancy, redundancyhold, holdpressure
Application NoteHydrogen Deuterium Exchange Coupledwith SELECT SERIES Cyclic IMS MassSpectrometer for Higher WorkflowEfficiencyLindsay Morrison, Malcolm Anderson, Alexandre GomesWaters CorporationFor research use only. Not for use in diagnostic procedures. AbstractHydrogen deuterium exchange is increasingly employed in the study of larger proteins and...
Key words
cyclic, cyclicims, imshdx, hdxracetrack, racetrackhydrogen, hydrogendeuterium, deuteriumexchange, exchangeselect, selectseries, seriessynapt, synaptpeptides, peptidesundertaken, undertakenpeptide, peptideadc, adcexperiments
ACQUITY UPLC M-Class System with HDX TechnologyIntegration of Hydrogen Deuterium Exchange with High Resolution MSQUANT IF Y CHANGES IN P ROT EIN CONFORMAT ION W IT H CONFIDENC EThe Waters ® ACQUITY UPLC ® M-Class System with HDX Technology provides...
Key words
hdx, hdxuplc, uplcdeuterium, deuteriumprotein, proteinenzymate, enzymateacquity, acquitywaters, watersexchange, exchangeconformat, conformatclass, classchanges, changespegylated, pegylatedsyst, systproceeds, proceedspepsin
PRESSURIZED ONLINE PEPSIN DIGESTION OF PROTEINS FOR HYDROGEN/DEUTERIUM EXCHANGEMASS SPECTROMETRYJing Fang, Ying Qing Yu, Rose Lawler, Keith Fadgen, Michael Eggertson, and Asish ChakrabortyWaters Corp., Milford, MA, USRESULTS AND DISCUSSIONINTRODUCTIONOnline pepsin digestion has been widely utilized in hydrogen/deuterium exchange massspectrometry (HDX...
Key words
digestion, digestioncoverage, coveragepeptides, peptidessequence, sequencequench, quenchdeuterium, deuteriumdesalting, desaltingexchange, exchangebradykinin, bradykininpepsin, pepsinhdx, hdxhold, holdnumber, numberpressurized, pressurizeddeuterated
 

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Pressurized Online Pepsin Digestion of mAb IgG2 forHydrogen Deuterium Exchange Mass SpectrometryJing Fang, Ying Qing Yu, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITSINTRODUCTIONAn enhanced-pressure (up to ~ 15,000 psi),Pepsin digestion is an integral part of hydrogen deuterium exchange masssustainable...
Key words
digestion, digestionpepsin, pepsinchain, chainhdx, hdxquench, quenchdeuterium, deuteriumcoverage, coveragepeptides, peptidesheavy, heavybradykinin, bradykininsequence, sequencelight, lightredundancy, redundancyhold, holdpressure
Application NoteHydrogen Deuterium Exchange Coupledwith SELECT SERIES Cyclic IMS MassSpectrometer for Higher WorkflowEfficiencyLindsay Morrison, Malcolm Anderson, Alexandre GomesWaters CorporationFor research use only. Not for use in diagnostic procedures. AbstractHydrogen deuterium exchange is increasingly employed in the study of larger proteins and...
Key words
cyclic, cyclicims, imshdx, hdxracetrack, racetrackhydrogen, hydrogendeuterium, deuteriumexchange, exchangeselect, selectseries, seriessynapt, synaptpeptides, peptidesundertaken, undertakenpeptide, peptideadc, adcexperiments
ACQUITY UPLC M-Class System with HDX TechnologyIntegration of Hydrogen Deuterium Exchange with High Resolution MSQUANT IF Y CHANGES IN P ROT EIN CONFORMAT ION W IT H CONFIDENC EThe Waters ® ACQUITY UPLC ® M-Class System with HDX Technology provides...
Key words
hdx, hdxuplc, uplcdeuterium, deuteriumprotein, proteinenzymate, enzymateacquity, acquitywaters, watersexchange, exchangeconformat, conformatclass, classchanges, changespegylated, pegylatedsyst, systproceeds, proceedspepsin
PRESSURIZED ONLINE PEPSIN DIGESTION OF PROTEINS FOR HYDROGEN/DEUTERIUM EXCHANGEMASS SPECTROMETRYJing Fang, Ying Qing Yu, Rose Lawler, Keith Fadgen, Michael Eggertson, and Asish ChakrabortyWaters Corp., Milford, MA, USRESULTS AND DISCUSSIONINTRODUCTIONOnline pepsin digestion has been widely utilized in hydrogen/deuterium exchange massspectrometry (HDX...
Key words
digestion, digestioncoverage, coveragepeptides, peptidessequence, sequencequench, quenchdeuterium, deuteriumdesalting, desaltingexchange, exchangebradykinin, bradykininpepsin, pepsinhdx, hdxhold, holdnumber, numberpressurized, pressurizeddeuterated
 

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