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Application NoteImproved Chromatographic Analysis ofOligonucleotides with ACQUITY PREMIEROligonucleotide BEH C18 ColumnsMartin Gilar, Zhimin Li, Jason MacLeanWaters Corporation AbstractThis application note illustrates sources of oligonucleotide loss due to adsorption on metalliccolumn hardware (frits). Solutions, columns, and best practices for improved oligonucleotideanalysis are...
Key words
oligonucleotide, oligonucleotideoligonucleotides, oligonucleotidesmiser, misersurfaces, surfacesmetallic, metallicfrit, fritadsorption, adsorptionuplc, uplcacquity, acquitypremier, premierstainless, stainlesssteel, steelhps, hpsmaxpeak, maxpeakoligodeoxythymidine
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideuplc, uplcacquity, acquityrnai, rnaiost, ostduplex, duplexpromass, promassduplexes, duplexessirna, sirnawaters, watersqda, qdarna, rnaanalysis, analysispairing
Evaluation of Alternative Ion-pairing Reagents in the Analysisof Oligonucleotides with the ACQUITY QDa DetectorRobert E. Birdsall and Ying Qing YuWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■Method compatibility with alternativeElectrospray ionization- (ESI) based analyses of oligonucleotides areion-pairing reagentsoften performed with buffers comprised...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideacquity, acquityqda, qdapairing, pairingalternative, alternativereagents, reagentshfip, hfipdibutylamine, dibutylaminepolyt, polytbutylamine, butylaminedetector, detectorost, ostevaluation, evaluationion
ION-PAIRING SYSTEMS FOR REVERSED-PHASECHROMATOGRAPHY OF OLIGONUCLEOTIDESSean M. McCarthy, Ananya Dubey, Martin GilarWaters Corporation, 34 Maple Street, Milford, MA 01757INTRODUCTIONCOMPARISON OF RESOLUTION FORION-PAIRING SYSTEMSSynthetic oligonucleotides are produced using highly efficient solid phase processes which havebeen optimized to produce very high yields,...
Key words
hfip, hfiphaa, haacapacity, capacitytpaa, tpaatea, teaoligonucleotides, oligonucleotidesmodifiers, modifierspeak, peakpairing, pairingacetate, acetatedmbaa, dmbaaheteropolymers, heteropolymershomomeric, homomericyield, yieldheteromeric
 

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Application NoteImproved Chromatographic Analysis ofOligonucleotides with ACQUITY PREMIEROligonucleotide BEH C18 ColumnsMartin Gilar, Zhimin Li, Jason MacLeanWaters Corporation AbstractThis application note illustrates sources of oligonucleotide loss due to adsorption on metalliccolumn hardware (frits). Solutions, columns, and best practices for improved oligonucleotideanalysis are...
Key words
oligonucleotide, oligonucleotideoligonucleotides, oligonucleotidesmiser, misersurfaces, surfacesmetallic, metallicfrit, fritadsorption, adsorptionuplc, uplcacquity, acquitypremier, premierstainless, stainlesssteel, steelhps, hpsmaxpeak, maxpeakoligodeoxythymidine
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideuplc, uplcacquity, acquityrnai, rnaiost, ostduplex, duplexpromass, promassduplexes, duplexessirna, sirnawaters, watersqda, qdarna, rnaanalysis, analysispairing
Evaluation of Alternative Ion-pairing Reagents in the Analysisof Oligonucleotides with the ACQUITY QDa DetectorRobert E. Birdsall and Ying Qing YuWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■Method compatibility with alternativeElectrospray ionization- (ESI) based analyses of oligonucleotides areion-pairing reagentsoften performed with buffers comprised...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideacquity, acquityqda, qdapairing, pairingalternative, alternativereagents, reagentshfip, hfipdibutylamine, dibutylaminepolyt, polytbutylamine, butylaminedetector, detectorost, ostevaluation, evaluationion
ION-PAIRING SYSTEMS FOR REVERSED-PHASECHROMATOGRAPHY OF OLIGONUCLEOTIDESSean M. McCarthy, Ananya Dubey, Martin GilarWaters Corporation, 34 Maple Street, Milford, MA 01757INTRODUCTIONCOMPARISON OF RESOLUTION FORION-PAIRING SYSTEMSSynthetic oligonucleotides are produced using highly efficient solid phase processes which havebeen optimized to produce very high yields,...
Key words
hfip, hfiphaa, haacapacity, capacitytpaa, tpaatea, teaoligonucleotides, oligonucleotidesmodifiers, modifierspeak, peakpairing, pairingacetate, acetatedmbaa, dmbaaheteropolymers, heteropolymershomomeric, homomericyield, yieldheteromeric
 

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