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Application NoteImproved Chromatographic Analysis ofOligonucleotides with ACQUITY PREMIEROligonucleotide BEH C18 ColumnsMartin Gilar, Zhimin Li, Jason MacLeanWaters Corporation AbstractThis application note illustrates sources of oligonucleotide loss due to adsorption on metalliccolumn hardware (frits). Solutions, columns, and best practices for improved oligonucleotideanalysis are...
Key words
oligonucleotide, oligonucleotideoligonucleotides, oligonucleotidesmiser, misersurfaces, surfacesmetallic, metallicfrit, fritadsorption, adsorptionuplc, uplcacquity, acquitypremier, premierstainless, stainlesssteel, steelhps, hpsmaxpeak, maxpeakoligodeoxythymidine
Application NoteIntact Mass Confirmation Analysis on theBioAccord LC-MS System for a Variety ofExtensively Modified OligonucleotidesCatalin E. Doneanu, Jonathan Fox, Emma Harry, Chris Knowles, Ying Qing Yu, JosephFredette, Weibin ChenWaters CorporationFor research use only. Not for use indiagnostic procedures. AbstractThis application note...
Key words
oligonucleotides, oligonucleotidesmodified, modifiedoligonucleotide, oligonucleotideunmodified, unmodifiedisotopic, isotopicdeconvolution, deconvolutionbioaccord, bioaccordmodel, modelheavily, heavilyextensively, extensivelyppt, pptoligo, oligomodifications, modificationssection, sectioncompliance
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideuplc, uplcacquity, acquityrnai, rnaiost, ostduplex, duplexpromass, promassduplexes, duplexessirna, sirnawaters, watersqda, qdarna, rnaanalysis, analysispairing
Evaluation of Alternative Ion-pairing Reagents in the Analysisof Oligonucleotides with the ACQUITY QDa DetectorRobert E. Birdsall and Ying Qing YuWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■Method compatibility with alternativeElectrospray ionization- (ESI) based analyses of oligonucleotides areion-pairing reagentsoften performed with buffers comprised...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideacquity, acquityqda, qdapairing, pairingalternative, alternativereagents, reagentshfip, hfipdibutylamine, dibutylaminepolyt, polytbutylamine, butylaminedetector, detectorost, ostevaluation, evaluationion
 

Similar PDF

Application NoteImproved Chromatographic Analysis ofOligonucleotides with ACQUITY PREMIEROligonucleotide BEH C18 ColumnsMartin Gilar, Zhimin Li, Jason MacLeanWaters Corporation AbstractThis application note illustrates sources of oligonucleotide loss due to adsorption on metalliccolumn hardware (frits). Solutions, columns, and best practices for improved oligonucleotideanalysis are...
Key words
oligonucleotide, oligonucleotideoligonucleotides, oligonucleotidesmiser, misersurfaces, surfacesmetallic, metallicfrit, fritadsorption, adsorptionuplc, uplcacquity, acquitypremier, premierstainless, stainlesssteel, steelhps, hpsmaxpeak, maxpeakoligodeoxythymidine
Application NoteIntact Mass Confirmation Analysis on theBioAccord LC-MS System for a Variety ofExtensively Modified OligonucleotidesCatalin E. Doneanu, Jonathan Fox, Emma Harry, Chris Knowles, Ying Qing Yu, JosephFredette, Weibin ChenWaters CorporationFor research use only. Not for use indiagnostic procedures. AbstractThis application note...
Key words
oligonucleotides, oligonucleotidesmodified, modifiedoligonucleotide, oligonucleotideunmodified, unmodifiedisotopic, isotopicdeconvolution, deconvolutionbioaccord, bioaccordmodel, modelheavily, heavilyextensively, extensivelyppt, pptoligo, oligomodifications, modificationssection, sectioncompliance
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideuplc, uplcacquity, acquityrnai, rnaiost, ostduplex, duplexpromass, promassduplexes, duplexessirna, sirnawaters, watersqda, qdarna, rnaanalysis, analysispairing
Evaluation of Alternative Ion-pairing Reagents in the Analysisof Oligonucleotides with the ACQUITY QDa DetectorRobert E. Birdsall and Ying Qing YuWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■■■Method compatibility with alternativeElectrospray ionization- (ESI) based analyses of oligonucleotides areion-pairing reagentsoften performed with buffers comprised...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideacquity, acquityqda, qdapairing, pairingalternative, alternativereagents, reagentshfip, hfipdibutylamine, dibutylaminepolyt, polytbutylamine, butylaminedetector, detectorost, ostevaluation, evaluationion
 

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