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Application NotePharma & BiopharmaDevelopment of a Rapid APTS SamplePreparation Workflow for N-GlycanRelease and LabelingAuthorsAndres Guerrero, John Yan,Ace G. Galermo, Tom Rice,Jim Torrence, Justin Hyche,Ted Haxo, Sergey Vlasenko,and Aled JonesAgilent Technologies, Inc.,Hayward, CAAarti JashnaniBristol-Myers SquibbRedwood City, CAAbstractThe N-glycan moieties of biotherapeutics...
Key words
apts, aptsgly, glyglycans, glycansglycan, glycanexpress, expressadvancebio, advancebiolabeling, labelingrfu, rfucleanup, cleanuplabeled, labeledenbrel, enbrelsialidase, sialidasemabthera, mabtherahilic, hilicglykoprep
Application NotePharma & BiopharmaComparison of Common FluorescentLabels for LC/MS Analysis ofReleased N-Linked GlycansAuthorsJohn Yan, Andres Guerrero,Steven Mast, Emily Dale,Ted Haxo, and Aled JonesAgilent Technologies, Inc.Abstract•Analysis of N-glycans released from biotherapeutics frequently relies on theaddition of a label/fluorophore.•Using N-glycans released from...
Key words
instantpc, instantpcglycans, glycansglycan, glycanfluorescence, fluorescencenhac, nhacprocainamide, procainamideinstantab, instantablabeling, labelingmabthera, mabtherabpi, bpilabeled, labeledreleased, releasedglykoprep, glykopreptof, tofhilic
Application NotePharm & BiopharmaDevelopment of a Rapid 2-AB SamplePreparation Workflow for N-GlycanRelease and LabelingAuthorsVaishali Sharma,Andres Guerrero, John Yan,Aled Jones, Michael Kimzey,Emily Dale, Ted Haxo, andSergey VlasenkoAgilent Technologies, Inc.AbstractThe characterization of N-glycans is essential to the development of biotherapeutics.Typically, enzymatically released...
Key words
glycan, glycangly, glyglycans, glycansglykoprep, glykoprepnistmab, nistmablabeling, labelingmabthera, mabtheraadvancebio, advancebioexpress, expressnhac, nhacfluorescence, fluorescenceenbrel, enbrelcleanup, cleanupdeglycosylation, deglycosylationreductant
Application NotePharma & BiopharmaStreamlined Workflows for N-GlycanAnalysis of Biotherapeutics UsingAgilent AdvanceBio Gly-X InstantPCand 2-AB Express Sample Preparationwith LC/FLD/MSAuthorsJohn Yan and Aled JonesAgilent Technologies, Inc.AbstractThis Application Note describes the preparation and analysis of releasedN-glycans from biotherapeutic glycoproteins using two labels, InstantPC...
Key words
glycan, glycaninstantpc, instantpcglycans, glycansgly, glyglycosylamine, glycosylaminelabeling, labelingnhac, nhacminutes, minutesfld, fldrel, relunits, unitsglycosylation, glycosylationacquisition, acquisitionlabeled, labeledfluorescence
 

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Application NotePharma & BiopharmaDevelopment of a Rapid APTS SamplePreparation Workflow for N-GlycanRelease and LabelingAuthorsAndres Guerrero, John Yan,Ace G. Galermo, Tom Rice,Jim Torrence, Justin Hyche,Ted Haxo, Sergey Vlasenko,and Aled JonesAgilent Technologies, Inc.,Hayward, CAAarti JashnaniBristol-Myers SquibbRedwood City, CAAbstractThe N-glycan moieties of biotherapeutics...
Key words
apts, aptsgly, glyglycans, glycansglycan, glycanexpress, expressadvancebio, advancebiolabeling, labelingrfu, rfucleanup, cleanuplabeled, labeledenbrel, enbrelsialidase, sialidasemabthera, mabtherahilic, hilicglykoprep
Application NotePharma & BiopharmaComparison of Common FluorescentLabels for LC/MS Analysis ofReleased N-Linked GlycansAuthorsJohn Yan, Andres Guerrero,Steven Mast, Emily Dale,Ted Haxo, and Aled JonesAgilent Technologies, Inc.Abstract•Analysis of N-glycans released from biotherapeutics frequently relies on theaddition of a label/fluorophore.•Using N-glycans released from...
Key words
instantpc, instantpcglycans, glycansglycan, glycanfluorescence, fluorescencenhac, nhacprocainamide, procainamideinstantab, instantablabeling, labelingmabthera, mabtherabpi, bpilabeled, labeledreleased, releasedglykoprep, glykopreptof, tofhilic
Application NotePharm & BiopharmaDevelopment of a Rapid 2-AB SamplePreparation Workflow for N-GlycanRelease and LabelingAuthorsVaishali Sharma,Andres Guerrero, John Yan,Aled Jones, Michael Kimzey,Emily Dale, Ted Haxo, andSergey VlasenkoAgilent Technologies, Inc.AbstractThe characterization of N-glycans is essential to the development of biotherapeutics.Typically, enzymatically released...
Key words
glycan, glycangly, glyglycans, glycansglykoprep, glykoprepnistmab, nistmablabeling, labelingmabthera, mabtheraadvancebio, advancebioexpress, expressnhac, nhacfluorescence, fluorescenceenbrel, enbrelcleanup, cleanupdeglycosylation, deglycosylationreductant
Application NotePharma & BiopharmaStreamlined Workflows for N-GlycanAnalysis of Biotherapeutics UsingAgilent AdvanceBio Gly-X InstantPCand 2-AB Express Sample Preparationwith LC/FLD/MSAuthorsJohn Yan and Aled JonesAgilent Technologies, Inc.AbstractThis Application Note describes the preparation and analysis of releasedN-glycans from biotherapeutic glycoproteins using two labels, InstantPC...
Key words
glycan, glycaninstantpc, instantpcglycans, glycansgly, glyglycosylamine, glycosylaminelabeling, labelingnhac, nhacminutes, minutesfld, fldrel, relunits, unitsglycosylation, glycosylationacquisition, acquisitionlabeled, labeledfluorescence
 

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