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Identification of NISTmAb Charge Variants by Fully Automated Capillary Isoelectric Focusing and Mass Spectrometry

Applications | 2019 | Agilent TechnologiesInstrumentation
LC/TOF, LC/HRMS, LC/MS, LC/MS/MS, Capillary electrophoresis
Industries
Pharma & Biopharma
Manufacturer
Agilent Technologies
Key words
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Application Note Pharma & Biopharma Monoclonal Antibody Charge Variants Identification by Fully Automated Capillary Isoelectric Focusing – Mass Spectrometry Authors Tony Wang, James Xia, and Rena Wang CMP Scientific Corp, Brooklyn, NY, USA Jens Meixner Agilent Technologies, Inc. Waldbronn, Germany…
Key words
cief, ciefdaratumumab, daratumumabcounts, countscytc, cytccmp, cmpdara, daracharge, chargeemass, emasscapillary, capillarycatholyte, catholyteisoelectric, isoelectricammonium, ammoniumhydroxide, hydroxidesheath, sheathfocusing
Charge Heterogeneity Analysis of Intact Infliximab Using CESI-MS and Neutral OptiMS Cartridge Fang Wang1, Tingting Li1, Esme Candish2 1 SCIEX, Brea, CA; 2SCIEX, Framingham, MA; Key Features • Capillary zone electrophoresis (CZE) separation and Mass spectrometric (MS) identification of charge…
Key words
charge, chargevariants, variantscesi, cesiinfliximab, infliximabvariant, variantcief, ciefcze, czemonoclonal, monoclonalelectrophoresis, electrophoresisstressed, stressedoptims, optimsseparation, separationunstressed, unstressedantibody, antibodycapillary
Separation of Native Monoclonal Antibodies and Identification of Charge Variants: Teamwork of the Agilent 3100 OFFGEL Fractionator, Agilent 2100 Bioanalyzer and Agilent LC/MS Systems Application Note Biosimilar and Biotherapeutics Authors Abstract Suresh Babu CV and Charge variants due to post-translational…
Key words
offgel, offgelcharge, chargevariants, variantsisoelectric, isoelectricmab, mabfractions, fractionsheterogeneity, heterogeneityfractionation, fractionationsubjected, subjectedbioanalyzer, bioanalyzercounts, countsthiourea, thioureafocusing, focusingcharacterization, characterizationnative
Comparison of Different Brands of Carrier Ampholytes for Monoclonal Antibody Charge Heterogeneity Analysis by Capillary Isoelectric Focusing Application Note Biotherapeutics & Biosimilars Author Abstract Christian Wenz This Application Note compares the performance of four commercially available Agilent Technologies, Inc. brands…
Key words
aeslyte, aeslytecief, ciefpharmalyte, pharmalyteisoform, isoformisoelectric, isoelectricservalyt, servalytbrands, brandsmau, maucharge, chargeheterogeneity, heterogeneitycapillary, capillaryisoforms, isoformsobserved, observedfocusing, focusingobtained
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