Pesticides and biocides are widely used in agriculture, building materials, and urban gardening to control pests and protect crops and public health. While much attention has been paid to pesticide residues in food, water, and outdoor environments, far less is known about their presence inside homes, where people spend the majority of their time. A new RECETOX study published in Indoor Environments addresses this gap by examining pesticides detected in house dust and their relevance for human exposure.&nbsp;The study reveals that Czech household dust commonly contains multiple pesticide residues, highlighting the persistence of both historic and current chemical use indoors. Modern pesticides were detected in 93% of homes, with two fungicides (carbendazim and propiconazole) most frequently found, while compounds related to the long‑banned pesticide DDT were present in dust from all homes, and were at higher levels in homes over 40 years old. Homes located closer to agricultural areas showed higher levels of current‑use pesticides in spring, indicating an influence of nearby farming activities. &nbsp;House dust acts as a sink for chemicals transported indoors from outdoor sources as well as from indoor use. Many pesticides can persist in dust for long periods, leading to continuous exposure in indoor spaces. This is particularly important for young children, who have higher exposure to dust from playing on the floor and mouthing toys and objects. The research underscores that indoor environments are not isolated from their surroundings. Understanding what ends up in house dust is a crucial step toward protecting public health and developing more comprehensive approaches to managing chemical risks in everyday life.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_Vyzkum_pesticidu_v_domacim_prachu_opomijena_cesta_lidske_expozice_a9a089fd2f.jpg" alt="RECETOX: Pesticides in House Dust: An Overlooked Pathway of Human Exposure" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_RECETOX_Vyzkum_pesticidu_v_domacim_prachu_opomijena_cesta_lidske_expozice_a9a089fd2f.jpg 195w," sizes="100vw" width="738" height="591"><blockquote><h4>Original Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S2950362026000214?via%3Dihub">Legacy and current-use pesticides in indoor settled dust and association with building age and seasonality</a></h4>Paula Marcinekova, Ondřej Mikeš, Simona Rozárka Jílková, Petr Šenk, Jakub Martiník, Jan Böhm, Petra Borilova Linhartova, Lenka Andrýsková, Jana Klánová, Lisa MelymukIndoor Environ., 2026, 3, 100169<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.indenv.2026.100169">https://doi.org/10.1016/j.indenv.2026.100169</a>&nbsp;<h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Indoor pesticide exposure is increasingly recognized as a significant contributor to pollutant exposure in residential spaces. This study investigates concentrations of legacy organochlorine pesticides (OCPs) and current-use pesticides (CUPs) in settled dust from 116 homes in the Czech Republic, in the context of their seasonal variability, relationships with building characteristics, geospatial factors, and indoor dust bacteriome diversity and composition. CUPs were detected in dust in 93% of homes, with carbendazim (84.5%), propiconazole (49.1%), atrazine (24.1%), and tebuconazole (11.2%) detected most frequently. OCPs were found in all homes. Propiconazole concentrations were significantly higher in homes with adjacent gardens, potentially reflecting its use as a biocide in outdoor building materials. CUP concentrations had clear seasonal trends, with peak levels in spring and summer, corresponding to pesticide application periods, particularly in rural environments. OCP concentrations were substantially higher in houses over 40 years old, indicating the persistence of residues from past indoor uses. While pesticide presence was ubiquitous, exposure estimates for children, under both average and high-exposure scenarios, were well below U.S. EPA reference doses (RfDs) for individual compounds; however, most homes had 3–11 pesticides in dust, raising the possibility of mixture effects. No significant associations, using a Spearman rank correlation matrix, were observed between pesticide concentrations and indoor dust bacteriome diversity and composition. These findings highlight the presence of pesticides in homes, including those in urban areas, driven by both current and historical sources, and reinforce the importance of monitoring to better understand potential exposure pathways.<h4>Instrumentation</h4><h5>1. LC–MS/MS analysis of CUPs</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=27&amp;mainauthorItems=10&amp;search=1290+Series+Gradient">Agilent 1290 Series Gradient HPLC</a></li><li>Columns:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1701">Phenomenex Luna C18</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=34&amp;mainauthorItems=50&amp;search=SecurityGuard+C18">Phenomenex SecurityGuard C18</a></li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=55&amp;search=QTrap+5500%2B">SCIEX QTrap 5500+</a> (triple quadrupole MS, ESI+)</li></ul><h5>2. GC–MS/MS analysis of OCPs</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?instrumentationItems=1&amp;mainauthorItems=10&amp;search=8890A">Agilent 8890A GC</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?instrumentationItems=19&amp;mainauthorItems=7&amp;search=Rxi-5Sil-MS">Restek Rxi-5Sil-MS capillary column</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=7000D">Agilent 7000D triple quadrupole MS</a></li></ul>

Pesticides in House Dust: An Overlooked Pathway of Human Exposure

This technical note presents a practical troubleshooting strategy for one of the most common issues in liquid chromatography (LC): shifting retention times. The approach is based on a systematic distinction between system-related (physical) and chromatographic (chemical) causes.A key diagnostic parameter used throughout the process is the T₀ (dead time), representing an unretained peak that passes through the column without interaction.<h3>Key Concept: T₀ (Dead Time / Unretained Peak)</h3>T₀ is defined as the retention time of a compound that does not interact with the stationary phase and therefore elutes directly with the mobile phase.<h4>How to Determine T₀</h4>T₀ can be determined experimentally by adding a marker compound to the system:<ul><li>Uracil</li><li>Potassium nitrate</li></ul>These compounds are added to a blank or method and produce a measurable unretained peak used for troubleshooting.<h3>Step 1: Evaluate T₀ Behavior</h3>The first and most important step is to determine whether T₀ is constant or variable.<ul><li>Case A: T₀ is Variable (Shifting) → Indicates a system-related (physical) problem &nbsp;</li><li>Case B: T₀ is Constant → Indicates a chromatographic or chemical problem</li></ul><h3>System-Related Issues (T₀ is Variable)</h3>If T₀ is shifting, the problem is likely related to the LC system hardware or physical parameters. This is especially true when all peaks shift together in the chromatogram.<h3>Possible Causes</h3><ul><li>Incorrect Flow Rate</li><li>Pump Malfunction<ul><li>Inconsistent or unstable flow delivery can cause retention time variability.</li></ul></li><li>Incorrect Column Length<ul><li>Using a column with different dimensions than expected.</li></ul></li><li>Solvent Delivery Issues<ul><li>Empty solvent reservoir (system running dry)</li><li>Blockages or restrictions (e.g., frits, solvent lines)</li></ul></li><li>Tubing or System Volume Changes<ul><li>Changes in tubing configuration</li><li>Differences in system dwell (swell) volume</li></ul></li></ul><h3>Chromatographic / Chemical Issues (T₀ is Constant)</h3>If T₀ remains stable but only specific peaks shift, the issue is likely related to chemistry or column conditions.<h4>Possible Causes</h4><ul><li>Incorrect Stationary Phase<ul><li>Column with same dimensions but different chemistry (e.g., different silica or bonded phase)</li></ul></li><li>Ionizable Analytes Ionizable compounds exist in:<ul><li>Neutral form - More hydrophobic, retained longer on a reversed-phase column</li><li>Ionized form</li></ul>Impact of pH Small pH changes in the mobile phase can significantly affect:<ul><li>Ionization state</li><li>Retention time of specific analytes</li></ul></li><li>Mobile Phase Issues<ul><li>Old solvents</li><li>Evaporation of volatile components</li><li>Changes in composition</li></ul></li><li>4. Temperature Variations<ul><li>Temperature affects analyte–stationary phase interactions</li><li>Even small fluctuations can shift retention times</li></ul></li></ul><h3>Step-by-Step Troubleshooting Workflow</h3><ol><li>Add T₀ Marker<ol><li>Use uracil or potassium nitrate</li></ol></li><li>Monitor T₀<ol><li>Determine whether it is constant or shifting</li></ol></li><li>If T₀ is Variable → Check System<ol><li>Flow rate settings</li><li>Pump performance</li><li>Solvent levels and lines</li><li>Column dimensions</li><li>Tubing and system volume</li></ol></li><li>If T₀ is Constant → Check Chemistry<ol><li>Column phase</li><li>Mobile phase composition and pH</li><li>Solvent quality</li><li>Temperature stability</li><li>Nature of analytes (ionizable vs. non-ionizable)</li></ol></li></ol><h3>Discussion and Practical Insight</h3>This troubleshooting approach simplifies a complex problem by dividing it into two main categories:<ul><li>Physical/system-related</li><li>Chemical/chromatographic</li></ul>The use of T₀ as a diagnostic tool allows rapid identification of the problem origin, reducing unnecessary trial-and-error steps.Particular attention should be paid to ionizable compounds, as they are highly sensitive to pH variations, which can lead to significant and selective retention time shifts.<h3>Conclusion</h3>Retention time shifts in LC can be efficiently diagnosed by evaluating the behavior of the T₀ (unretained peak):<ul><li>Variable T₀ → System issue</li><li>Constant T₀ → Chromatographic/chemical issue</li></ul>By following this structured approach, analysts can quickly narrow down the root cause and implement targeted corrective actions.

Troubleshooting Retention Time Shifts in Liquid Chromatography 

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RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

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Phenomenex Kinetex F5 Core-Shell Columns

Combine core-shell performance, multiple retention mechanisms and the Kinetex F5 column’s ability to be run in a variety of separation modes.

Maximize Retention Interactions and PerformanceCombine core-shell performance, multiple retention mechanisms and the Kinetex F5 column’s ability to be run in a variety of separation modes (reversed phase, SFC, 2D LC, and 100% aqueous) and you now have an impeccable method development tool at your disposal.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>100 % Aqueous Mobile Phases Stable</li><li>5 Mechanisms of Retention and Selectivity</li><li>USP: L43</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_F5_Core_Shell_HPLC_Column_188a3f510f.webp" alt="Phenomenex: Kinetex F5 Core-Shell HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_F5_Core_Shell_HPLC_Column_188a3f510f.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: null %</li><li>Particle Size (μm): 1.7, 2.6, 5</li><li>Recommended Use: Highly reproducible pentafluorophenylpropyl phase, exceptional for halogenated, conjugated, isomeric, or highly polar compounds.</li></ul><h4>F5 - A Novel Selectivity For Tough Separations</h4>This pentafluorophenyl propyl column provides a very high degree of steric selectivity to separate structural isomers. The electronegative fluorine groups offer high selectivity for cationic compounds.<h4>Dependable F5 Selectivity</h4>While older pentafluorophenyl phases (PFP, PFPP, F5, etc.) are based on existing bonding techniques and technologies that promote irreproducibility. The Kinetex F5 was meticulously designed by Phenomenex R&amp;D and its customers, to provide consistently accurate and high performance results.<h4>Take Advantage of Multiple Interactive Mechanisms</h4>The multiple interactive mechanisms of the Kinetex F5 (pentafluorophenyl ) column successfully separate methoxybenzene isomers, while the Kinetex C18 column, which has minimal bonding interactions, cannot separate the methoxybenzene isomers. This demonstrates that columns that rely primarily on hydrophobic interactions may not be the first choice for the separation of isomeric compounds and a column with multiple interactive mechanisms may be required.<blockquote>Stationary phase Kinetex F5 is provided in these column types: analytical and preparative</blockquote><h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Kinetex Phenyl-Hexyl Core-Shell Columns

The Kinetex Phenyl-Hexyl column combines the performance gains of the Kinetex Core-Shell technology with balanced hydrophobic and aromatic selectivity.

Performance and Balanced SelectivityThe Kinetex Phenyl-Hexyl column combines the performance gains of the Kinetex Core-Shell technology with balanced hydrophobic and aromatic selectivity.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems*</li><li>Balanced Hydrophobic and Aromatic Selectivity</li><li>Available in Three Particle Sizes</li><li>USP:L11</li></ul><figure class="image"><img style="aspect-ratio:193/210;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_Phenyl_Hexyl_Core_Shell_HPLC_Column_a2f28d943f.jpg" alt="Phenomenex: Kinetex phenyl-hexyl Core-Shell HPLC column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_Phenyl_Hexyl_Core_Shell_HPLC_Column_a2f28d943f.jpg 143w," sizes="100vw" width="193" height="210"></figure><ul><li>Carbon Load: 10.0, null %, 10.0 %</li><li>Particle Size (μm): 1.7, 2.6, 5</li><li>Recommended Use: Allows for greater retention and separation of aromatic hydrocarbons</li></ul><h4>Diverse Selectivity For Closely Related Compounds</h4>Aromatic and moderate hydrophobic selectivity result in the great retention and separation of aromatic hydrocarbons<h4>Phenyl Selectivity with Balanced Hydrophobic Retention</h4>Retain and separate a wide class of compounds with the unique and balanced selectivity of the Kinetex Phenyl-hexyl. The phenyl-hexyl column consistently provides reproducible and transient interactions that increase polar selectivity and deliver good chromatographic peak shape.<blockquote>Stationary phase Kinetex Phenyl-hexyl is provided in these column types: analytical and preparative</blockquote><h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Luna PFP(2) HPLC Columns

The Luna PFP(2) is a pentafluorophenyl phase with strongly electronegative fluorine groups to provide polar selectivity for cationic compounds.

The Luna PFP(2) is a pentafluorophenyl phase with strongly electronegative fluorine groups to provide polar selectivity for cationic compounds, while the rigid bonded phase is a good steric selector.<ul><li>Extremely discerning for halogenated‚ aromatic and conjugated compounds</li><li>Provides orthogonal selectivity even using traditional reversed phase mobile phase systems</li><li>PFP Ultra pure, metal-free silica (99.99 % purity) (USP: L43)/li&gt;</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_PFP_2_HPLC_Column_9750ad436d.webp" alt="Phenomenex: Luna PFP(2) HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_PFP_2_HPLC_Column_9750ad436d.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5</li><li>Recommended Use: Separation of aromatic, halogenated, and closely related compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Superior Particle Smoothness</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Superior_Particle_Smoothness_of_Luna_C18_f44c5b3028.jpg" alt="Phenomenex: Superior Particle Smoothness of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Superior_Particle_Smoothness_of_Luna_C18_f44c5b3028.jpg 245w," sizes="100vw" width="1385" height="442"><h4>4 Mechanisms of Interaction</h4>Luna® PFP(2) columns provide unique selectivity for highly polar compounds, complex natural products, isomers and other closely related compounds. This is achieved by using a propyl linked pentafluorophenyl, which provides multiple retention mechanisms unique to typical reversed phase medias.<ul><li>Hydrogen Bonding</li><li>Dipole-Dipole Interactions</li><li>Aromatic and π-π Interactions</li><li>Hydrophobic</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_PFP_2_Column_selectivity_c7b9591ed6.jpg" alt="Phenomenex: Luna PFP(2) Column selectivity." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_PFP_2_Column_selectivity_c7b9591ed6.jpg 193w," sizes="100vw" width="863" height="698"><h4>Common Applications:</h4><ul><li>Positional isomers, Geometric isomers, Taxanes, Aflatoxins</li></ul><blockquote>Stationary phase Luna PFP(2) is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna PFP(2) HPLC Microbore, Minibore, MidBore, Analytical and Semi-preparative Columns are available with these parameters:</h5>Microbore Columns<ul><li>Column length (mm): 150</li><li>Column ID (mm): 1</li><li>Particle size (µm): 3</li></ul>Minibore Columns<ul><li>Column length (mm): 30, 50, 100, 150</li><li>Column ID (mm): 2</li><li>Particle size (µm): 3, 5</li></ul>MidBore Columns<ul><li>Column length (mm): 50, 150</li><li>Column ID (mm): 3</li><li>Particle size (µm): 3, 5</li></ul>Analytical Columns<ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 4.6</li><li>Particle size (µm): 3, 5</li></ul>Semi-Prep Columns<ul><li>Column length (mm): 250</li><li>Column ID (mm): 10</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex: Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Luna Phenyl-Hexyl HPLC Columns

The Luna Phenyl Hexyl is our most hydrophobic phenyl column and it will also provide good hydrogen accepting functionality for acidic retention.

The Luna Phenyl Hexyl is our most hydrophobic phenyl column and it will also provide good hydrogen accepting functionality for acidic retention.<ul><li>Extensive line of rugged particle sizes</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>Phenyl-Hexyl Ultra-pure, metal-free silica (99.99% purity) (USP: L11)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_phenyl_hexyl_HPLC_Column_e0aa55de27.jpg" alt="Phenomenex: Luna phenyl-hexyl HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_phenyl_hexyl_HPLC_Column_e0aa55de27.jpg 245w," sizes="100vw" width="599" height="309"><ul><li>Carbon Load: 17.5, 17.5 %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5, 10, 15</li><li>Recommended Use: Separation of aromatic and closely related compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Engineered for Stability</h4>Luna Phenyl-Hexyl columns provide separations not achievable on C18 or C8 columns; such as increased retention for polar‚ aromatic compounds as well as reversals in analyte elution order. Luna Phenyl-Hexyl columns are a reproducible‚ extremely stable phenyl phase.<h4>Common Applications:</h4><ul><li>Oxacillin, Captopril, Chlorpheniramine, Pseudoephedrine, Methadone Hydrochloride Oral Concentration</li></ul><blockquote>Stationary phase Luna Phenyl-Hexyl is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna Phenyl-Hexyl HPLC Microbore, Minibore, MidBore, Analytical and Semi-preparative Columns are available with these parameters:</h5>Microbore Columns<ul><li>Column length (mm): 50, 100, 150</li><li>Column ID (mm): 1</li><li>Particle size (µm): 3, 5</li></ul>Minibore Columns<ul><li>Column length (mm): 30, 50, 100, 150, 250</li><li>Column ID (mm): 2</li><li>Particle size (µm): 3, 5</li></ul>MidBore Columns<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 3</li><li>Particle size (µm): 3, 5</li></ul>Analytical Columns<ul><li>Column length (mm): 30, 50, 75, 100, 150, 250</li><li>Column ID (mm): 4.6</li><li>Particle size (µm): 3, 5</li></ul>Semi-Prep Columns<ul><li>Column length (mm): 250</li><li>Column ID (mm): 10</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Thermo Scientific Accucore Biphenyl Reversed Phase HPLC Columns

Choose Thermo Scientific™ Accucore™ Biphenyl LC Columns for fast and reliable separation of critical pairs and isomers.

Choose Thermo Scientific™ Accucore™ Biphenyl LC Columns for fast and reliable separation of critical pairs and isomers. These columns provide unique selectivity for aromatic and moderately polar analytes. Their optimized packing offers a rugged platform for a variety of matrices. Based on Core Enhanced Technology, Accucore liquid chromatography reversed phase columns provide fast, high-resolution separations without the elevated backpressures seen using sub-2μm particles.<ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Mixed-mode selectivity for aromatic and moderately polar analytes</li><li>Enhanced Pi-pi interactions with aromatics</li><li>Excellent lot-to-lot reproducibility</li></ul>Trust your separation of critical pairs and isomers to Accucore Biphenyl LC Columns. These columns offer fast run times, excellent robustness, and consistent reproducibility, and provide the perfect balance of polar analyte retention and hydrophobic analyte elution.<h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC/UHPLC</li><li>Max. Pressure: 8700 psi (600 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Biphenyl</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L11</li><li>Carbon Load: 6%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul>

Thermo Scientific Accucore Phenyl-Hexyl HPLC Columns

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.

Offer unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Accucore™ Phenyl-Hexyl LC Columns.Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations without the elevated backpressures required by sub-2μm particles.<ul><li>Fast separations with excellent resolution and sensitivity</li><li>Lower backpressures so that UHPLC is not required</li><li>Mixed-Mode Selectivity for Aromatic and Moderately Polar Analytes</li><li>Enhanced Pi-pi Interactions with Aromatics</li><li>Moderate Hydrophobicity</li></ul><h4>Specifications</h4><ul><li>Endcapped: Yes</li><li>For Use With: HPLC</li><li>Max. Pressure: 14500 psi (1000 bar)</li><li>Packing Material: Spherical, Solid Core, Ultrapure Silica</li><li>Particle Shape: Spherical Solid Core</li><li>Stationary Phase: Phenyl/Hexyl</li><li>Surface Area: 130 m²/g</li><li>Temperature: 70°C</li><li>USP Type: L11</li><li>Carbon Load: 5%</li><li>Phase: Reversed Phase</li><li>Pore Size: 80 Å</li><li>pH: 2 to 8</li></ul>

Products from category Phenyl HPLC columns - page 3

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