Pesticides and biocides are widely used in agriculture, building materials, and urban gardening to control pests and protect crops and public health. While much attention has been paid to pesticide residues in food, water, and outdoor environments, far less is known about their presence inside homes, where people spend the majority of their time. A new RECETOX study published in Indoor Environments addresses this gap by examining pesticides detected in house dust and their relevance for human exposure.&nbsp;The study reveals that Czech household dust commonly contains multiple pesticide residues, highlighting the persistence of both historic and current chemical use indoors. Modern pesticides were detected in 93% of homes, with two fungicides (carbendazim and propiconazole) most frequently found, while compounds related to the long‑banned pesticide DDT were present in dust from all homes, and were at higher levels in homes over 40 years old. Homes located closer to agricultural areas showed higher levels of current‑use pesticides in spring, indicating an influence of nearby farming activities. &nbsp;House dust acts as a sink for chemicals transported indoors from outdoor sources as well as from indoor use. Many pesticides can persist in dust for long periods, leading to continuous exposure in indoor spaces. This is particularly important for young children, who have higher exposure to dust from playing on the floor and mouthing toys and objects. The research underscores that indoor environments are not isolated from their surroundings. Understanding what ends up in house dust is a crucial step toward protecting public health and developing more comprehensive approaches to managing chemical risks in everyday life.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_Vyzkum_pesticidu_v_domacim_prachu_opomijena_cesta_lidske_expozice_a9a089fd2f.jpg" alt="RECETOX: Pesticides in House Dust: An Overlooked Pathway of Human Exposure" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_RECETOX_Vyzkum_pesticidu_v_domacim_prachu_opomijena_cesta_lidske_expozice_a9a089fd2f.jpg 195w," sizes="100vw" width="738" height="591"><blockquote><h4>Original Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S2950362026000214?via%3Dihub">Legacy and current-use pesticides in indoor settled dust and association with building age and seasonality</a></h4>Paula Marcinekova, Ondřej Mikeš, Simona Rozárka Jílková, Petr Šenk, Jakub Martiník, Jan Böhm, Petra Borilova Linhartova, Lenka Andrýsková, Jana Klánová, Lisa MelymukIndoor Environ., 2026, 3, 100169<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.indenv.2026.100169">https://doi.org/10.1016/j.indenv.2026.100169</a>&nbsp;<h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Indoor pesticide exposure is increasingly recognized as a significant contributor to pollutant exposure in residential spaces. This study investigates concentrations of legacy organochlorine pesticides (OCPs) and current-use pesticides (CUPs) in settled dust from 116 homes in the Czech Republic, in the context of their seasonal variability, relationships with building characteristics, geospatial factors, and indoor dust bacteriome diversity and composition. CUPs were detected in dust in 93% of homes, with carbendazim (84.5%), propiconazole (49.1%), atrazine (24.1%), and tebuconazole (11.2%) detected most frequently. OCPs were found in all homes. Propiconazole concentrations were significantly higher in homes with adjacent gardens, potentially reflecting its use as a biocide in outdoor building materials. CUP concentrations had clear seasonal trends, with peak levels in spring and summer, corresponding to pesticide application periods, particularly in rural environments. OCP concentrations were substantially higher in houses over 40 years old, indicating the persistence of residues from past indoor uses. While pesticide presence was ubiquitous, exposure estimates for children, under both average and high-exposure scenarios, were well below U.S. EPA reference doses (RfDs) for individual compounds; however, most homes had 3–11 pesticides in dust, raising the possibility of mixture effects. No significant associations, using a Spearman rank correlation matrix, were observed between pesticide concentrations and indoor dust bacteriome diversity and composition. These findings highlight the presence of pesticides in homes, including those in urban areas, driven by both current and historical sources, and reinforce the importance of monitoring to better understand potential exposure pathways.<h4>Instrumentation</h4><h5>1. LC–MS/MS analysis of CUPs</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=27&amp;mainauthorItems=10&amp;search=1290+Series+Gradient">Agilent 1290 Series Gradient HPLC</a></li><li>Columns:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1701">Phenomenex Luna C18</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=34&amp;mainauthorItems=50&amp;search=SecurityGuard+C18">Phenomenex SecurityGuard C18</a></li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=55&amp;search=QTrap+5500%2B">SCIEX QTrap 5500+</a> (triple quadrupole MS, ESI+)</li></ul><h5>2. GC–MS/MS analysis of OCPs</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?instrumentationItems=1&amp;mainauthorItems=10&amp;search=8890A">Agilent 8890A GC</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?instrumentationItems=19&amp;mainauthorItems=7&amp;search=Rxi-5Sil-MS">Restek Rxi-5Sil-MS capillary column</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=7000D">Agilent 7000D triple quadrupole MS</a></li></ul>

Pesticides in House Dust: An Overlooked Pathway of Human Exposure

This technical note presents a practical troubleshooting strategy for one of the most common issues in liquid chromatography (LC): shifting retention times. The approach is based on a systematic distinction between system-related (physical) and chromatographic (chemical) causes.A key diagnostic parameter used throughout the process is the T₀ (dead time), representing an unretained peak that passes through the column without interaction.<h3>Key Concept: T₀ (Dead Time / Unretained Peak)</h3>T₀ is defined as the retention time of a compound that does not interact with the stationary phase and therefore elutes directly with the mobile phase.<h4>How to Determine T₀</h4>T₀ can be determined experimentally by adding a marker compound to the system:<ul><li>Uracil</li><li>Potassium nitrate</li></ul>These compounds are added to a blank or method and produce a measurable unretained peak used for troubleshooting.<h3>Step 1: Evaluate T₀ Behavior</h3>The first and most important step is to determine whether T₀ is constant or variable.<ul><li>Case A: T₀ is Variable (Shifting) → Indicates a system-related (physical) problem &nbsp;</li><li>Case B: T₀ is Constant → Indicates a chromatographic or chemical problem</li></ul><h3>System-Related Issues (T₀ is Variable)</h3>If T₀ is shifting, the problem is likely related to the LC system hardware or physical parameters. This is especially true when all peaks shift together in the chromatogram.<h3>Possible Causes</h3><ul><li>Incorrect Flow Rate</li><li>Pump Malfunction<ul><li>Inconsistent or unstable flow delivery can cause retention time variability.</li></ul></li><li>Incorrect Column Length<ul><li>Using a column with different dimensions than expected.</li></ul></li><li>Solvent Delivery Issues<ul><li>Empty solvent reservoir (system running dry)</li><li>Blockages or restrictions (e.g., frits, solvent lines)</li></ul></li><li>Tubing or System Volume Changes<ul><li>Changes in tubing configuration</li><li>Differences in system dwell (swell) volume</li></ul></li></ul><h3>Chromatographic / Chemical Issues (T₀ is Constant)</h3>If T₀ remains stable but only specific peaks shift, the issue is likely related to chemistry or column conditions.<h4>Possible Causes</h4><ul><li>Incorrect Stationary Phase<ul><li>Column with same dimensions but different chemistry (e.g., different silica or bonded phase)</li></ul></li><li>Ionizable Analytes Ionizable compounds exist in:<ul><li>Neutral form - More hydrophobic, retained longer on a reversed-phase column</li><li>Ionized form</li></ul>Impact of pH Small pH changes in the mobile phase can significantly affect:<ul><li>Ionization state</li><li>Retention time of specific analytes</li></ul></li><li>Mobile Phase Issues<ul><li>Old solvents</li><li>Evaporation of volatile components</li><li>Changes in composition</li></ul></li><li>4. Temperature Variations<ul><li>Temperature affects analyte–stationary phase interactions</li><li>Even small fluctuations can shift retention times</li></ul></li></ul><h3>Step-by-Step Troubleshooting Workflow</h3><ol><li>Add T₀ Marker<ol><li>Use uracil or potassium nitrate</li></ol></li><li>Monitor T₀<ol><li>Determine whether it is constant or shifting</li></ol></li><li>If T₀ is Variable → Check System<ol><li>Flow rate settings</li><li>Pump performance</li><li>Solvent levels and lines</li><li>Column dimensions</li><li>Tubing and system volume</li></ol></li><li>If T₀ is Constant → Check Chemistry<ol><li>Column phase</li><li>Mobile phase composition and pH</li><li>Solvent quality</li><li>Temperature stability</li><li>Nature of analytes (ionizable vs. non-ionizable)</li></ol></li></ol><h3>Discussion and Practical Insight</h3>This troubleshooting approach simplifies a complex problem by dividing it into two main categories:<ul><li>Physical/system-related</li><li>Chemical/chromatographic</li></ul>The use of T₀ as a diagnostic tool allows rapid identification of the problem origin, reducing unnecessary trial-and-error steps.Particular attention should be paid to ionizable compounds, as they are highly sensitive to pH variations, which can lead to significant and selective retention time shifts.<h3>Conclusion</h3>Retention time shifts in LC can be efficiently diagnosed by evaluating the behavior of the T₀ (unretained peak):<ul><li>Variable T₀ → System issue</li><li>Constant T₀ → Chromatographic/chemical issue</li></ul>By following this structured approach, analysts can quickly narrow down the root cause and implement targeted corrective actions.

Troubleshooting Retention Time Shifts in Liquid Chromatography 

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RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

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Thermo Scientific Dionex IonPac AS15 IC Columns

Determine trace-level concentrations of inorganic anions and low-molecular-weight organic acids in high-purity water matrices with the Thermo Scientific Dionex IonPac AS15 Capillary, Analytical & Guard Columns.

Determine trace-level concentrations of inorganic anions and low-molecular-weight organic acids in high-purity water matrices with the Thermo Scientific™ Dionex™ IonPac™ AS15 Capillary, Analytical &amp; Guard Columns. Specifically designed for analysis of trace anions in high-purity water matrices used by the semiconductor and power generation industries, the column delivers high sensitivity and superior resolution of early-eluting anions (e.g., fluoride, glycolate, acetate, and formate). The column is optimized for selective at an operating temperature of 30°C, and it is ideal for use with large-loop injections.<h5>Available in Multiple Formats</h5><ul><li>Dionex IonPac AS15-5μm (3x150mm) column for high efficiency, fast analysis (15 min)</li><li>Dionex IonPac AS15-9μm (2x250mm, 4x250mm, and 0.4x250mm) columns for higher-capacity applications</li><li>Dionex IonPac AS15 capillary column (0.4x250mm) delivers the performance of the standard bore version but requires only 1% of the eluent flow rate, which helps reduce operating costs</li></ul><h5>For Best Performance</h5><ul><li>Use the Thermo Scientific™ Dionex™ IonPac™ AC15 Concentrator column for ultratrace (part per trillion) analyses</li><li>Use a Dionex eluent generator for simplified eluent preparation</li><li>Use the Thermo Scientific™ Dionex™ Anion Self-Regenerating Suppressor (ASRS™ 300) or the Thermo Scientific™ Dionex™ Anion Capillary Electrolytic Suppressor (ACES™ 300) with the Dionex IonPac AS15 column for eluent suppression</li></ul><h4>Specifications</h4><ul><li>Anion Exchange Capacity: 225 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 225 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS15</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS16 IC Columns

Measure trace perchlorate and other highly polarizable anions quickly and accurately in a variety of water matrices with the Thermo Scientific Dionex IonPac AS16 IC Columns.

Measure trace perchlorate and other highly polarizable anions quickly and accurately in a variety of water matrices with the Thermo Scientific™ Dionex™ IonPac™ AS16 IC Columns . This column simplifies the determination of polarizable anions, including thiosulfate, iodide, thiocyanate, and perchlorate using an isocratic hydroxide eluent. It is ideally suited for determination of trace perchlorate in drinking water in accordance with U.S. EPA Methods 314.0 and 314.1 (Primary Method).<h5>Designed for Simple, Sensitive Analysis</h5><ul><li>Easily measure trace concentrations of perchlorate in drinking water, surface water, and ground water matrices using a large-loop injection</li><li>Separate hydrophobic anions (iodide, thiocyanate, and thiosulfate) in less than 20 min</li><li>Ideal for polyvalent anions, including polyphosphates and polycarboxylates</li><li>Ultralow hydrophobicity allows fast analysis of polarizable anions</li><li>Can be used in the second dimension of the 2D-IC method for the analysis of trace perchlorate (sub ppb) in drinking water</li></ul><h5>Available in Multiple Formats</h5><ul><li>Standard bore (4x250mm) and microbore (2x250mm) formats</li></ul><h4>Specifications</h4><ul><li>Anion Exchange Capacity: 170 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 170 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS16</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS17-C IC Columns

Quickly determine common inorganic anions in high-purity water matrices with the Thermo Scientific Dionex IonPac AS17-C column. 

Quickly determine common inorganic anions in high-purity water matrices with the Thermo Scientific™ Dionex IonPac AS17-C column. This low-capacity column is optimized for fast gradient separation of inorganic anions in simple sample matrices. It is also ideal for fast gradient separation of sulfur species, including sulfite, sulfate, and thiosulfate. The Dionex IonPac AS17C column is the recommended replacement for all Dionex IonPac AS17 column anion applications.<h5>Make Gradient Separations as Easy as Isocratic Runs</h5>The Dionex IonPac AS17-C column is designed to be used in conjunction with the Dionex Eluent Generator. The Dionex Eluent Generator automatically produces potassium hydroxide eluents from water, making gradient separations as easy as isocratic runs. Using the Dionex Eluent Generator under gradient conditions, common inorganic anions can easily be separated in a variety of sample matrices including high purity water, drinking water, wastewater, process streams, and scrubber solutions.<h5>Achieve Superior Chromatographic Performance</h5><ul><li>Fast gradient separation of inorganic anions in high purity water matrices</li><li>Easy suppression of hydroxide eluent to a very low background with the Thermo Scientific™ Dionex™ Anion Self-Regenerating Suppressor (ASRS™ 300) for trace level determinations (including fluoride) even with a large loop injection</li><li>Superior retention and quantification of fluoride, acetate, propionate, and formate</li><li>Meets performance requirements specified in U.S. EPA Method 300.0 (A)</li><li>Operates at ambient or elevated temperatures</li><li>Column selectivity is optimized for operating temperature of 30°C to ensure reproducible results</li><li>Compatible with Reagent-Free™ Ion Chromatography (RFIC™) systems</li><li>Compatible with organic solvents to enhance analyte solubility, modify column selectivity, or for effective column clean-up</li></ul>Note: The Thermo Scientific™ Dionex™ IonPac™ AS18 column is the recommended hydroxide-selective column for determination of common anions in diverse sample matrices.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 30 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 30 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS17-C</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

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News from LabRulezLCMS Library - Week 18, 2026