Pesticides and biocides are widely used in agriculture, building materials, and urban gardening to control pests and protect crops and public health. While much attention has been paid to pesticide residues in food, water, and outdoor environments, far less is known about their presence inside homes, where people spend the majority of their time. A new RECETOX study published in Indoor Environments addresses this gap by examining pesticides detected in house dust and their relevance for human exposure.&nbsp;The study reveals that Czech household dust commonly contains multiple pesticide residues, highlighting the persistence of both historic and current chemical use indoors. Modern pesticides were detected in 93% of homes, with two fungicides (carbendazim and propiconazole) most frequently found, while compounds related to the long‑banned pesticide DDT were present in dust from all homes, and were at higher levels in homes over 40 years old. Homes located closer to agricultural areas showed higher levels of current‑use pesticides in spring, indicating an influence of nearby farming activities. &nbsp;House dust acts as a sink for chemicals transported indoors from outdoor sources as well as from indoor use. Many pesticides can persist in dust for long periods, leading to continuous exposure in indoor spaces. This is particularly important for young children, who have higher exposure to dust from playing on the floor and mouthing toys and objects. The research underscores that indoor environments are not isolated from their surroundings. Understanding what ends up in house dust is a crucial step toward protecting public health and developing more comprehensive approaches to managing chemical risks in everyday life.&nbsp;<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_Vyzkum_pesticidu_v_domacim_prachu_opomijena_cesta_lidske_expozice_a9a089fd2f.jpg" alt="RECETOX: Pesticides in House Dust: An Overlooked Pathway of Human Exposure" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_RECETOX_Vyzkum_pesticidu_v_domacim_prachu_opomijena_cesta_lidske_expozice_a9a089fd2f.jpg 195w," sizes="100vw" width="738" height="591"><blockquote><h4>Original Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S2950362026000214?via%3Dihub">Legacy and current-use pesticides in indoor settled dust and association with building age and seasonality</a></h4>Paula Marcinekova, Ondřej Mikeš, Simona Rozárka Jílková, Petr Šenk, Jakub Martiník, Jan Böhm, Petra Borilova Linhartova, Lenka Andrýsková, Jana Klánová, Lisa MelymukIndoor Environ., 2026, 3, 100169<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.indenv.2026.100169">https://doi.org/10.1016/j.indenv.2026.100169</a>&nbsp;<h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Indoor pesticide exposure is increasingly recognized as a significant contributor to pollutant exposure in residential spaces. This study investigates concentrations of legacy organochlorine pesticides (OCPs) and current-use pesticides (CUPs) in settled dust from 116 homes in the Czech Republic, in the context of their seasonal variability, relationships with building characteristics, geospatial factors, and indoor dust bacteriome diversity and composition. CUPs were detected in dust in 93% of homes, with carbendazim (84.5%), propiconazole (49.1%), atrazine (24.1%), and tebuconazole (11.2%) detected most frequently. OCPs were found in all homes. Propiconazole concentrations were significantly higher in homes with adjacent gardens, potentially reflecting its use as a biocide in outdoor building materials. CUP concentrations had clear seasonal trends, with peak levels in spring and summer, corresponding to pesticide application periods, particularly in rural environments. OCP concentrations were substantially higher in houses over 40 years old, indicating the persistence of residues from past indoor uses. While pesticide presence was ubiquitous, exposure estimates for children, under both average and high-exposure scenarios, were well below U.S. EPA reference doses (RfDs) for individual compounds; however, most homes had 3–11 pesticides in dust, raising the possibility of mixture effects. No significant associations, using a Spearman rank correlation matrix, were observed between pesticide concentrations and indoor dust bacteriome diversity and composition. These findings highlight the presence of pesticides in homes, including those in urban areas, driven by both current and historical sources, and reinforce the importance of monitoring to better understand potential exposure pathways.<h4>Instrumentation</h4><h5>1. LC–MS/MS analysis of CUPs</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=27&amp;mainauthorItems=10&amp;search=1290+Series+Gradient">Agilent 1290 Series Gradient HPLC</a></li><li>Columns:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1701">Phenomenex Luna C18</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=34&amp;mainauthorItems=50&amp;search=SecurityGuard+C18">Phenomenex SecurityGuard C18</a></li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=55&amp;search=QTrap+5500%2B">SCIEX QTrap 5500+</a> (triple quadrupole MS, ESI+)</li></ul><h5>2. GC–MS/MS analysis of OCPs</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?instrumentationItems=1&amp;mainauthorItems=10&amp;search=8890A">Agilent 8890A GC</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?instrumentationItems=19&amp;mainauthorItems=7&amp;search=Rxi-5Sil-MS">Restek Rxi-5Sil-MS capillary column</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=7000D">Agilent 7000D triple quadrupole MS</a></li></ul>

Pesticides in House Dust: An Overlooked Pathway of Human Exposure

This technical note presents a practical troubleshooting strategy for one of the most common issues in liquid chromatography (LC): shifting retention times. The approach is based on a systematic distinction between system-related (physical) and chromatographic (chemical) causes.A key diagnostic parameter used throughout the process is the T₀ (dead time), representing an unretained peak that passes through the column without interaction.<h3>Key Concept: T₀ (Dead Time / Unretained Peak)</h3>T₀ is defined as the retention time of a compound that does not interact with the stationary phase and therefore elutes directly with the mobile phase.<h4>How to Determine T₀</h4>T₀ can be determined experimentally by adding a marker compound to the system:<ul><li>Uracil</li><li>Potassium nitrate</li></ul>These compounds are added to a blank or method and produce a measurable unretained peak used for troubleshooting.<h3>Step 1: Evaluate T₀ Behavior</h3>The first and most important step is to determine whether T₀ is constant or variable.<ul><li>Case A: T₀ is Variable (Shifting) → Indicates a system-related (physical) problem &nbsp;</li><li>Case B: T₀ is Constant → Indicates a chromatographic or chemical problem</li></ul><h3>System-Related Issues (T₀ is Variable)</h3>If T₀ is shifting, the problem is likely related to the LC system hardware or physical parameters. This is especially true when all peaks shift together in the chromatogram.<h3>Possible Causes</h3><ul><li>Incorrect Flow Rate</li><li>Pump Malfunction<ul><li>Inconsistent or unstable flow delivery can cause retention time variability.</li></ul></li><li>Incorrect Column Length<ul><li>Using a column with different dimensions than expected.</li></ul></li><li>Solvent Delivery Issues<ul><li>Empty solvent reservoir (system running dry)</li><li>Blockages or restrictions (e.g., frits, solvent lines)</li></ul></li><li>Tubing or System Volume Changes<ul><li>Changes in tubing configuration</li><li>Differences in system dwell (swell) volume</li></ul></li></ul><h3>Chromatographic / Chemical Issues (T₀ is Constant)</h3>If T₀ remains stable but only specific peaks shift, the issue is likely related to chemistry or column conditions.<h4>Possible Causes</h4><ul><li>Incorrect Stationary Phase<ul><li>Column with same dimensions but different chemistry (e.g., different silica or bonded phase)</li></ul></li><li>Ionizable Analytes Ionizable compounds exist in:<ul><li>Neutral form - More hydrophobic, retained longer on a reversed-phase column</li><li>Ionized form</li></ul>Impact of pH Small pH changes in the mobile phase can significantly affect:<ul><li>Ionization state</li><li>Retention time of specific analytes</li></ul></li><li>Mobile Phase Issues<ul><li>Old solvents</li><li>Evaporation of volatile components</li><li>Changes in composition</li></ul></li><li>4. Temperature Variations<ul><li>Temperature affects analyte–stationary phase interactions</li><li>Even small fluctuations can shift retention times</li></ul></li></ul><h3>Step-by-Step Troubleshooting Workflow</h3><ol><li>Add T₀ Marker<ol><li>Use uracil or potassium nitrate</li></ol></li><li>Monitor T₀<ol><li>Determine whether it is constant or shifting</li></ol></li><li>If T₀ is Variable → Check System<ol><li>Flow rate settings</li><li>Pump performance</li><li>Solvent levels and lines</li><li>Column dimensions</li><li>Tubing and system volume</li></ol></li><li>If T₀ is Constant → Check Chemistry<ol><li>Column phase</li><li>Mobile phase composition and pH</li><li>Solvent quality</li><li>Temperature stability</li><li>Nature of analytes (ionizable vs. non-ionizable)</li></ol></li></ol><h3>Discussion and Practical Insight</h3>This troubleshooting approach simplifies a complex problem by dividing it into two main categories:<ul><li>Physical/system-related</li><li>Chemical/chromatographic</li></ul>The use of T₀ as a diagnostic tool allows rapid identification of the problem origin, reducing unnecessary trial-and-error steps.Particular attention should be paid to ionizable compounds, as they are highly sensitive to pH variations, which can lead to significant and selective retention time shifts.<h3>Conclusion</h3>Retention time shifts in LC can be efficiently diagnosed by evaluating the behavior of the T₀ (unretained peak):<ul><li>Variable T₀ → System issue</li><li>Constant T₀ → Chromatographic/chemical issue</li></ul>By following this structured approach, analysts can quickly narrow down the root cause and implement targeted corrective actions.

Troubleshooting Retention Time Shifts in Liquid Chromatography 

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RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

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Waters Charged Aerosol Detector (CAD)

Waters Charged Aerosol Detector (CAD) enables universal quantitation of non-volatile and semi-volatile compounds independent of UV activity, with wide dynamic range and seamless integration with Empower™ Software.

<h3>Expand Your Detection Range with a Detector Purpose-Built for Empower™ Software</h3>Empower™ Software–based laboratories require detection solutions that are easy to adopt, robust, and capable of accurately quantifying non-volatile and semi-volatile compounds—regardless of chemical structure or UV activity. The Waters Charged Aerosol Detector (CAD) is purposefully engineered to meet these demands, delivering universal, reliable detection fully integrated with Waters chromatography systems and Empower™ or MassLynx™ Software.With a wide dynamic range exceeding four orders of magnitude, the Waters CAD enables simultaneous quantitation of low-level, non-UV-active analytes, along with related impurities and excipients, in a single run—helping laboratories reduce method development time, increase productivity, and improve process control.<h4>Key Benefits</h4><ul><li>Universal detection without UV limitations Detect and quantify non-volatile and semi-volatile compounds independent of chromophore presence.</li><li>Wide dynamic range (&gt;4 orders of magnitude) Confidently quantify trace-level components and major constituents simultaneously.</li><li>Purpose-built for Empower™ Software Seamless integration into regulated workflows with Empower-based qualification and long-term software compatibility.</li><li>Compatible across Waters LC platforms Integrates easily with Alliance™, Arc™, ACQUITY™ Arc, ACQUITY™ H-Class, ACQUITY™ I-Class, and UPLC™ Systems.</li><li>Designed for robustness and ease of use Optimized hardware, simplified maintenance, and enhanced system protection features minimize downtime.</li></ul><h4>Technical Specifications</h4><h5>Detector Performance</h5><ul><li>Detector flow rate: 0.010–2.000 mL/min</li><li>Dynamic range: Greater than four orders of magnitude (four individual power values)</li><li>Sampling rate: Up to 200 Hz</li><li>Number of channels: Four (4), supported by four (4) independent background current channels</li></ul><h5>Temperature Control</h5><ul><li>Evaporation temperature:<ul><li>Ambient (off)</li><li>Controlled range: 10 °C to 100 °C (0.1 °C increments)</li><li>Minimum of 5 °C above ambient</li></ul></li></ul><h5>Gas Requirements</h5><ul><li>Inert gas supply: Nitrogen (recommended) or compressed air</li><li>Inlet pressure: 4.83–5.52 bar (483–552 kPa, 70.0–80.0 psi)</li><li>Gas consumption: Typically ≤ 5 L/min</li></ul><h5>Diagnostics and Monitoring</h5><ul><li>Diagnostic channels include:<ul><li>Nebulizer gas flow and pressure (psi)</li><li>Evaporator temperature (°C)</li><li>Ion trap voltage (V)</li><li>Charger current (µA)</li><li>Mixing chamber temperature (°C)</li></ul></li></ul><h5>Electrical and Physical Specifications</h5><ul><li>Power requirements: 100–240 VAC</li><li>Dimensions:<ul><li>Width: 34.3 cm (13.5 in)</li><li>Height: 20.8 cm (8.2 in)</li><li>Depth: 61.0 cm (24.0 in)</li></ul></li><li>Biocompatible: No</li></ul><h5>Software and System Compatibility</h5><ul><li>Optimized for Empower™ Software and MassLynx™ Software control</li><li>Robust chromatography data system (CDS) communications to minimize downtime</li><li>Backward compatibility to Empower™ 3 Feature Release 4 Service Release 3 (SR3)</li><li>Fully compatible with systems qualification and validation requirements in regulated environments</li></ul><h5>Seamless Method Transfer Across Laboratories</h5>The Waters CAD is designed to simplify method transfer within organizations and across external laboratories. It maintains method simplicity while adding enhanced tools for equivalency and reproducibility, reducing the need for revalidation when transitioning from legacy or third-party CAD solutions.The intuitive ACQUITY™ console control panel enables straightforward operation and simplifies replacement of outdated technology across laboratory networks.<h5>Designed for Ease of Use and Reliability</h5><ul><li>Single filter manifold with push/pull filter replacement</li><li>Easy-to-replace nebulizer for improved serviceability</li><li>Intelligent divert valve automatically sends flow to waste if gas pressure is lost or system errors occur</li><li>Enhanced leak detection and flood protection, safeguarding instruments in the event of CDS communication loss</li></ul><h5>Recommended Use</h5>Accurate quantitation in:<ul><li>HPLC, UHPLC, and UPLC separations</li><li>Preparative and purification workflows</li><li>Pharmaceutical QC and R&amp;D</li><li>Analysis of excipients, lipids, sugars, surfactants, and non-UV-active impurities</li></ul>

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Troubleshooting Retention Time Shifts in Liquid Chromatography

Pesticides in House Dust: An Overlooked Pathway of Human Exposure

News from LabRulezLCMS Library - Week 18, 2026