Meeting EU Drinking Water Standards: The Critical Role of Sample Prep in Trace Contaminant Analysis

<h3>Charged for Action: The Hidden Secrets of AEX Oligonucleotide Analysis</h3>Oligonucleotides—short, well-defined fragments of DNA or RNA—have become indispensable tools in modern life sciences. They underpin cutting-edge applications in molecular diagnostics, gene therapy, and pharmaceutical development. Before oligonucleotides can be confidently used in research or clinical settings, however, their quality must be rigorously assessed. Purity, sequence integrity, length distribution, and chemical modifications all play a decisive role in their performance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg" alt="KNAUER: Oligos Made Easy - Part 2: AEX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_2_45eee54fa0.jpg 245w," sizes="100vw" width="1534" height="711">Among the analytical techniques available today, anion exchange chromatography (AEX) stands out as a robust and highly informative method for characterizing oligonucleotides. By exploiting their intrinsic negative charge, AEX provides detailed insight into product composition and impurity profiles that are difficult to obtain by other approaches.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Part 1 of the Oligos series: A Practical Guide to Oligonucleotide Production – From Concept to Final Product</a></blockquote><h4>How Scientists Read Oligonucleotides Using Charge</h4>At the core of AEX lies a simple but powerful principle: oligonucleotides carry a negatively charged phosphate backbone. This charge increases in a predictable way with strand length and, to a lesser extent, sequence composition. Anion exchange chromatography uses this property to separate oligonucleotides based on differences in charge strength and interaction with a positively charged stationary phase.As a result, AEX has become one of the most widely used analytical tools for evaluating oligonucleotide quality. It allows scientists to distinguish full-length products from truncated species, degradation products, or closely related variants—often with single-nucleotide resolution.<h4>Why Ion Exchange Works: Binding, Retention, and Release</h4>Ion exchange chromatography (IEX) relies on electrostatic interactions between analytes and a charged stationary phase. In the case of oligonucleotides, anion exchange is used, where negatively charged molecules interact with positively charged functional groups on the resin—most commonly quaternary amines.The system consists of two main components:<ul><li>Stationary phase: A positively charged resin that binds anionic analytes.</li><li>Mobile phase: An aqueous buffer system, typically combined with a salt gradient, that controls both pH and elution strength.</li></ul>Retention in AEX is governed primarily by charge. Molecules with weaker overall charge interact less strongly with the stationary phase and elute earlier, while more highly charged oligonucleotides bind more tightly and require stronger elution conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 1 IEX overview including elution order dependency on analyte charge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_1_IEX_overview_including_elution_order_dependency_on_analyte_charge_3419ad3075.jpg 245w," sizes="100vw" width="1583" height="654"><h4>The Five-Step Workflow of Ion Exchange Chromatography</h4>AEX separations generally follow a well-established sequence of steps:<ol><li>Column equilibration The column is conditioned with the starting buffer until the baseline and pH are stable. This ensures that all charged sites on the resin are ready for interaction.</li><li>Sample loading Samples, prepared in the starting buffer, are introduced under conditions that promote binding of the target oligonucleotides while minimizing retention of unwanted impurities.</li><li>Washing The column is washed with the starting buffer to remove uncharged or weakly interacting components. The baseline stabilizes as these species are flushed away.</li><li>Elution Bound oligonucleotides are released using a salt or pH gradient. Increasing ionic strength introduces competing ions that displace the analytes from the resin. Weakly bound species elute first; strongly charged oligonucleotides elute later.</li><li>Regeneration and re-equilibration A high-salt buffer removes any remaining bound material. The column is then returned to starting conditions in preparation for the next analysis.</li></ol><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 2 Five steps of IEX procedure (in blue), outlined using an example chromatogram (in red)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_2_Five_steps_of_IEX_procedure_in_blue_outlined_using_an_example_chromatogram_in_red_38dd70b1b1.jpg 245w," sizes="100vw" width="1583" height="674"><h4>Why AEX Is Especially Effective for Oligonucleotides</h4>The repeating phosphate backbone of oligonucleotides gives rise to a strong and uniform anionic character across a wide pH range (typically pH 7–9). This makes oligonucleotides exceptionally well suited for anion exchange separations.Key factors influencing AEX behavior include:<ul><li>Charge density: Longer oligonucleotides carry more negative charges and therefore exhibit stronger retention.</li><li>Resin chemistry: Strong and weak anion exchangers differ in functional groups and pH stability, influencing selectivity and robustness.</li><li>Buffer composition: The choice of buffering ions and salt gradients directly affects elution strength and resolution.</li></ul>Salt gradients—commonly based on chloride ions—compete with the oligonucleotide backbone for binding sites on the resin. By carefully tuning gradient slope and composition, analysts can achieve highly reproducible and finely resolved separations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 3 Ionic interactions and pH influence on Anion Exchange Chromatography (AEX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_3_Ionic_interactions_and_p_H_influence_on_Anion_Exchange_Chromatography_AEX_612e231ba4.jpg 245w," sizes="100vw" width="1547" height="593"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 4 AEX buffer selection and gradient strategy (chromatogram with red buffer gradient curve)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_4_AEX_buffer_selection_and_gradient_strategy_chromatogram_with_red_buffer_gradient_curve_53fd1bb11b.jpg 245w," sizes="100vw" width="1600" height="327"><h4>AEX as the Gold Standard for Oligonucleotide Separation</h4>Anion exchange chromatography has become the method of choice for both analytical and preparative oligonucleotide workflows. Its advantages include:<ul><li>High resolving power: Closely related impurities, including n–1 or n–2 species, can often be separated and quantified.</li><li>Predictable retention behavior: In many cases, retention time correlates linearly with oligonucleotide length, simplifying identification.</li><li>Compatibility with modified oligonucleotides: Even chemically modified sequences retain their anionic nature, allowing AEX to resolve both products and related impurities.</li><li>Excellent control via gradient elution: Salt gradients provide precise tuning of selectivity and resolution.</li></ul>Typically, shorter or degraded oligonucleotides elute at lower ionic strength, while full-length products with higher charge density appear later in the chromatogram.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg" alt="KNAUER: Oligos Made Easy - Part 2 AEX: Figure 5 AEX Advantages and Challenges." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Oligos_Made_Easy_Part_2_AEX_Figure_5_AEX_Advantages_and_Challenges_7835521e34.jpg 245w," sizes="100vw" width="1535" height="707"><h4>Final Perspective: Mastering AEX with Confidence</h4>Anion exchange chromatography may appear complex at first, but with thoughtful optimization of pH, buffer composition, and gradient conditions, it becomes a highly precise and reliable tool for oligonucleotide analysis. When properly applied, AEX delivers sharp peaks, excellent resolution, and robust performance—even for challenging sequences and modifications.For laboratories developing or refining oligonucleotide workflows, AEX offers both analytical depth and operational confidence.<blockquote>If you would like to discuss AEX method development or optimization for oligonucleotides, feel free to contact us at sales@knauer.net. More insights into practical oligonucleotide analysis will follow in our “Oligos Made Easy” series.For technical questions or in-depth discussion, the author can be reached at kindler@knauer.net</blockquote>

Oligos Made Easy - Part 2: AEX

Although modern agriculture needs to be based on accurate real-time data on soil, plant, and environmental conditions, affordable, sufficiently sensitive, and robust monitoring devices for this purpose are currently missing. According to scientists, low-cost sensors based on new smart materials and printing technologies offer a suitable solution. The results of multidisciplinary research thus far and proposals for the development of these devices have been presented by researchers from Czechia, Great Britain, Spain, and the USA in a review article in the prestigious journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-68778-6#Abs1">Nature Communications</a>.“Sensor technologies for monitoring plant health are still in their infancy. Only simple sensors capable of monitoring basic parameters such as pH, soil moisture, or temperature are commonly available on the market. However, if farmers are to face current challenges, including climate change, and ensure sufficient food for humanity, they need much more effective tools that will enable them to monitor, for example, various pathogens, including bacteria and fungi. In our opinion, a suitable solution is a combination of new smart materials and printing for the production of inexpensive, durable, and scalable materials,” said the article’s first author, David Panáček from CATRIN, who worked on the study during his internship at Imperial College London.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg" alt="CATRIN: David Panáček" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Soucasne_senzory_nestaci_Vedci_navrhuji_novou_cestu_ke_sledovani_zdravi_rostlin_b4014952a3.jpg 208w," sizes="100vw" width="1536" height="1152">Sensors for monitoring plant health, for example, lag behind similar devices used in human medicine. According to the authors, advances in materials science, which will enable the development of scalable sensor technologies, play a key role in this potential shift. “Printing techniques, including inkjet printing, screen printing, aerosol printing, 3D printing, and direct laser writing, offer flexible ways to produce flexible sensors, large-area sensors, and sensors integrated directly into plants. The review article summarizes recent advances in printable low-dimensional materials for agricultural sensors, analyzes their physico-chemical properties in relation to sensor performance, and discusses key challenges and future opportunities requiring interdisciplinary integration,” said one of the corresponding authors, Michal Otyepka.The research brought together materials scientists, chemists, biologists, sensing experts, and printing technology specialists. Materials research and the use of graphene-based smart materials were led by scientists from CATRIN at Palacký University and VŠB-Technical University of Ostrava. Plant biologists from CATRIN contributed their knowledge of plant development, their responses to stress conditions, and the monitoring of their properties. Researchers from Imperial College London took care of the electronics, while representatives from<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/36"> CEITEC – VUT</a> focused on printing technologies. The team also included leading experts in sensing from the Catalan Institute of Nanoscience and Nanotechnology in Barcelona, and Joseph Wang of the University of California San Diego, the “father of sensing and electrochemistry,” also contributed his extensive knowledge.“This is a groundbreaking study on this topic, which has managed to attract some truly big names in their respective fields. Last but not least, the study demonstrates that interdisciplinarity at CATRIN gives rise to completely new and promising research topics, such as plant sensing and its use for studying plant-environment interactions and smart agriculture,” added another corresponding author, Nuria De Diego.

Current sensors are not good enough. Scientists propose a new way to monitor plant health

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The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

SIELC Alltesta Mini High-Pressure Switching HPLC Valve

The Alltesta™ Mini High-Pressure Switching Valve offers versatility and convenience for laboratory fluid management applications.

The Alltesta™ Mini High-Pressure Switching Valve offers versatility and convenience for laboratory fluid management applications. It features flexible flow switching at varying flow rates and can be used independently or as part of the <a href="https://lcms.labrulez.com/products/124">Alltesta™ Analyzer</a> or <a href="https://lcms.labrulez.com/products/1244">Alltesta™ Autosampler</a>.
With customizable options, including up to 10 available ports on the stator, the valve can be tailored to specific needs. It is built to handle pressures up to 5000 psi (350 bar) and boasts a fast 60 degree switch time of 200 milliseconds.
<h4>Specifications</h4>
<ul>
<li>Power requirements: USB type B</li>
<li>Dimensions (WHL): 63 x 46 x 128 mm / 2.48 x 1.81 x 5.04 in</li>
<li>Weight: 0.45 kg / 0.95 lb</li>
<li>Switching speed for 60 degree: 200 ms</li>
<li>Max pressure: 350 bar (5000 psi)</li>
<li>Ports style: 10-32 thread with conical 1/16″ tube compression type seal</li>
<li>Materials in contact with liquid: coated SS316, Vespel® or PEEK or PTFE</li>
</ul>
<h4>Available Configurations</h4>
<ul>
<li>6x2 Valve</li>
<li>7x6 Valve</li>
<li>10x9 Valve</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Mini_High_Pressure_Switching_Valve_Available_Configurations_72e3b13ea2.jpg" alt="SIELC: SIELC Alltesta™ Mini High-Pressure Switching Valve - Available Configurations."/>
<h4>Software</h4>
The Alltesta™ High-Pressure Switching Valve can be controlled:
<ul>
<li>directly via serial commands (option with greatest flexibility, Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our software, allowing to use the whole functionality of the unit without the need for automation (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Valve_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 6 Mb, <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Valve_Control_Windows_7.zip">Windows 7, 32/64 bit</a>, 127 Mb),</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Analyzer</a>)</li>
<li>operator may also simply press the button on the front of the device to switch it to the next position</li>
</ul>
Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.
<h4>Available Custom Options</h4>
<ul>
<li>A High-flow version of the valve (preparative) is available</li>
<li>A metal-free version of the valve is also available.</li>
</ul>
<h4>Valve Rotor Position Indication</h4>
LED color indicates the valve’s rotor position. By pressing the LED, the rotor can be advanced by one position. Depending on the valve configuration, the second press will either advance the rotor forward or backward. Position 1 – LED Green Position 2 – LED Navy Position 3 – LED Purple Position 4 – LED Blue Position 5 – LED Red Position 6 – LED White
<h4>Applications</h4>
<ul>
<li>Column Switching</li>
<li>Solvent Selection</li>
<li>Sample Injection</li>
<li>Sample Cleaning</li>
</ul>

SIELC Alltesta™ Mini LHS (Liquid Handling Station)

The Alltesta™ Mini Liquid Handling Station serves as an affordable, reliable solution for automated sample handling.

The Alltesta™ Mini Liquid Handling Station serves as an affordable, reliable solution for automated sample handling. With its compact design, proprietary alpha/beta mechanical structure, and adjustable operation speeds, it offers comprehensive control for quick and seamless integration with other equipment. The station comes equipped with a built-in sample shaking feature for in-vial sample mixing and extraction, and a needle washing station capable of accommodating up to four solvents.
Designed with Original Equipment Manufacturer (OEM) customization in mind, the Alltesta™ Mini Liquid Handling Station can be tailored to fit any system’s requirements. Custom needle lengths and trays enable the device to accommodate various types of vials. An array of integrated valves and a measuring syringe can be added to the device. Additional customization options, such as metal-free flow paths and customer-specified enclosure colors, are also available.
Comprehensive software integration support is provided to ensure smooth operation and functionality.
<h4>Specifications</h4>
<ul>
<li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li>
<li>Weight: 2.25 kg / 5 lb</li>
<li>Power: 24 V</li>
<li>Capacity: 48-vial tray or 96-well plate</li>
<li>Needle Wash: Inside and Outside</li>
<li>Communication: RS232 Virtual COM port via USB</li>
</ul>
<h4>Software</h4>
The Alltesta™ LHS can be fully controlled via serial commands. Alltesta™ protocol available upon request, please contact us at support@sielc.com. Serial communication drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.

SIELC Alltesta Mini Syringe Pump

Alltesta™ Ultra High Pressure Mini Syringe Pump is designed to produce a steady, high pressure, pulsation-free flow, so there is no need for bulky dampeners, mixers, and precision check valve arrangements.

Alltesta™ Ultra High Pressure Mini Syringe Pump is designed to produce a steady, high pressure, pulsation-free flow, so there is no need for bulky dampeners, mixers, and precision check valve arrangements. It can refill prior to each run in less than a minute, and can produce flow rates from as low 1 μL/min to as fast as 4000 μL/min with pressure up to 4000 psi (285 bar).
The Mini Syringe Pump allows for several communication options via its USB/Serial port. Just like the rest of our Alltesta™ line of devices, our serial protocol allows to control and automate every function of the device. We also provide free Windows-based software allowing full control of the device.
<h4>Specifications</h4>
<ul>
<li>Syringe volume: 5.8 mL</li>
<li>Power Requirements 24 VDC</li>
<li>Current Requirements Up to 1.5 A</li>
<li>Dimensions (WHL) 63x167x128 mm / 2.48×6.57×5.04 in</li>
<li>Weight 1.65 kg / 3.7 lb</li>
<li>Flow Rate Range 0.001 to 4 mL/min</li>
<li>Speed Accuracy 1% of full-scale</li>
<li>Linearity Full Range 0.001 to 4.0 mL/min</li>
<li>Pressure 4000 psi (285 bar) continuous</li>
<li>Pressure accuracy ±1 psi</li>
<li>Operating Temperature 0 to 40° C, non-condensing</li>
<li>Inlet/Outlet Thread 10-32 UNF compression type</li>
<li>Materials in contact with liquid SS316, PTFE, PEEK</li>
<li>Communication USB virtual serial port</li>
</ul>
<h4>Software</h4>
The Mini Syringe Pump can be controlled:
<ul>
<li>directly through your software via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> and <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a>.),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Pump_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 5Mb; <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Pump_Control_Windows_7.zip">Windows 7, 32/64bit</a>, 126Mb)</li>
</ul>
Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.
<h4>Available Custom Options</h4>
Metal-free version of the pump includes no metal contact pump head and can be operated up to 4000 psi.

Products from category HPLC accessories focused on HPLC distributed by LabRulez - page 1

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Current sensors are not good enough. Scientists propose a new way to monitor plant health

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Oligos Made Easy - Part 2: AEX