Advancing Research with the SARS-CoV-2 LC-MS Kit (RUO)

Detection and quantification of SARS-CoV-2 is critically important in clinical research to aid understanding of SARS-CoV-2. The SARS-CoV-2 LC-MS Kit (RUO) enables the direct detection and quantification of SARS-CoV-2 Nucleocapsid (NCAP) proteotypic peptides at low concentrations. Stable Isotope Standards with Capture by Anti-Peptide Antibodies (SISCAPA) are used to provide the analytical sensitivity improvements required to detect the low levels of viral peptides from complex digested samples. The Andrew+ robot was used to streamline and automate the sample preparation process, minimizing hands-on preparation time and reducing operator error. The ACQUITY UPLC I-Class with ACQUITY Premier Column technology allows for the rapid separation of the target peptides, with the Xevo TQ-XS mass spectrometer providing analytically sensitive and selective detection of viral peptides operating in multiple reaction monitoring (MRM) detection mode.

The SARS-CoV-2 LC-MS Kit (RUO) allows for the quantification of viral proteins to be used alongside infection biomarkers to enable investigation of the impacts of the virus in longitudinal research studies.

For Research Use Only. Not for use in diagnostic procedures.

Presenter: Dominic Foley (Principal Scientist, Waters Corporation)

Dominic is a Principal Scientist working at Waters Mass Spectrometry Headquarters in Wilmslow, UK. Having joined the company in September 2013, he has specialized in the development of LC-MS/MS clinical research applications, with expertise in steroid hormone analysis and protein/peptide quantification. In his role, he also collaborates with clinical customers in the development and implementation of LC-MS/MS methods in their laboratories.

Dominic has worked in separation sciences for 14 years, following his ambition of improving the quality of healthcare. He started his career with ICON Development Solutions, a CRO in the north of the UK, where he worked as a LC-MS/MS method development and validation scientist supporting DMPK studies in drug discovery. In 2012, he joined Thermo Fisher Scientific to work in their Chromatography R&D team as a Technical Support Specialist, working on the development of new chromatography and sample preparation products.

Dominic received his BSc (Hons) in Biochemistry from the University of Manchester in 2006. He has presented his work at conferences around the world and has several publications in both the Microchemical Journal and the Journal of Chromatography A.