Avoid System Contamination with Ion-Pair-Free Analysis of Oligonucleotides by LC and RapidFire HILIC MS

LC/MS methods for the analysis of oligonucleotides have traditionally been based on ion-pairing reverse-phase (IP-RP) chromatography because these approaches generally deliver good separation and MS response in negative mode. However, considering that many ion-pairing reagents can present a memory effect which negatively impacts the performance of the system in positive mode, IP-RP methods can be burdensome for mixed-use systems and push many laboratories to seek chromatographic alternatives.

In this webinar, we present high-quality MS analysis of a wide variety of oligonucleotide samples without the use of ion-pairing reagents, on both LC and RapidFire 6545XT AdvanceBio QTOF configurations. These methods leverage Agilent HILIC-Z resins and MS-friendly, ammonium acetate-based, mobile phases, which allow for subsequent positive mode use of the system with little to no flushing or hardware changes.

Presenter: Peter Rye, PhD (LC/MS Applications Scientist, Agilent Technologies, Inc.)

Peter is a pre-sales Application Engineer with Agilent Technologies. He has been with Agilent for 10 years developing LC, RapidFire, and mass spec methods on a wide range of molecules. Prior to Agilent, Peter worked in analytical development groups for BioTove and BIOCIUS, the original companies that developed the RapidFire platform. In his spare time, you can find Peter relaxing with his wife and kids in Beverly Massachusetts, trying to fix the many problems with his home, or staying active outdoors.