HPLC troubleshooting basics: Peak Issues
Key Learning Points:
- Signal to noise improvement
- Unknown/ ghost peaks/ carryover Peaks
- Split peaks/ peak fronting/ double peaks
- Peak Tailing/ broad peaks/ flat peaks
- Irreproducible peak heights and peak areas
Presenter: Dr. Helen Whitby (Technical Specialist, Phenomenex United Kingdom)
Helen graduated from the University of Manchester with a First-Class Bachelor of Science with honors in Chemistry and subsequently completed her PhD also at the University of Manchester in synthetic organic chemistry specializing in analogues of Shikimic acid to treat malaria. Following her PhD, Helen moved to Chicago as a Post-doctoral researcher at the University of Illinois in Chicago to research on electron-deficient divalent reactive intermediates, specifically nitrenium ions, for the development of antiviral agents to treat Ebola.
After finishing her post doc, Helen joined Phenomenex UK as a technical sales consultant and spent 10 years as the Bulk Media and Preparative specialist for the UK and Ireland, supporting large purification companies with their production scale applications. In her 2 years as the HPLC product manager, Helen supported the UK team in all aspects of HPLC. Helen joined the Technical Department in 2017 as a technical specialist with a focus on the rapidly expanding bio industry and this is where her interest and expertise within the technical department remain today.
