Combining Evosep One with state of the art SWATH acquisition
In the ever lasting effort to boost the number of protein identified in MS-based proteomic experiments, it has become clear that Data Independent Analysis (DIA), combined with short gradients, has become a winning combination.
The steep development over the last years, have positioned DIA as an important acquisition strategy to generate record high identifications with short gradients on the Evosep One.
Join this webinar to explore how our customers have used Evosep One to generate record high IDs per minute for large-scale sample cohorts.
Combining Evosep One with state of the art SWATH acquisition
- Presenter: Yves Le Blanc (Associate Chief Research Scientist, SCIEX)
- Presenter: Ihor Batruch (Technical Product Manager at Sciex)
Accurate and reproducible compound identification from complex mixtures is ultimately the goal of any LC-MSMS analysis. To achieve this on a large number of analytes, Data Independent Analysis (DIA) methods have emerged as MSMS technique that can reliably detect the response of analytes over their entire chromatographic peak. Combination of these techniques with high performance LC separation provides higher confidence in compound identification.
Here we present the combination of rapid LC analysis provided by the Evosep system, along with SWATH acquisition approaches. The first approach focussed on Scanning-SWATH, over small mass range, in combination with the Evosep workflow of 200 and 100spd. Using 1 amu window and fast sample turn around, maximum coverage can be achieved with high confidence through replicate analysis.
The second approach focused on protein identification and quantification achievable with a ZenoTOF 7600 system in SWATH acquisition mode coupled to Evosep operated at workflows of 200, 100, 60 and 30 SPD across different HeLa digest sample loads. Performance improvements of a novel Zeno SWATH acquisition strategy relative to SWATH acquisition on a prototype ZenoTOF 7600 system will also be evaluated. A preliminary look at how these combined technologies can improve sample throughput as well compound coverage with be presented.
