ASMS Virtual Mass Spectrometry Users Meeting

The countdown to our virtual user meeting has begun. We cannot wait to share how our cutting-edge MS technology solutions can take your scientific discoveries to the next level.

Bringing together experts from across the industry, our exciting program will help you maximize the full potential of your mass spectrometer. You will also get a front row seat as we launch the latest addition to our MS instrument family.

Join us on June 1, where you can learn how the latest MS technologies, software and services are solving complex analytical challenges, no matter the application.

The Evolution of Xevo

Discover the Next Generation of Xevo

• Jayne Kirk (Principal Product Marketing Manager, Waters Corporation)

Abstract coming...

Performance Evaluation of Next Generation Q-Tof for Biotherapeutic Product Quality Assays

• Andy Mahan (Associate Professor, Pharmaceutical Sciences; Executive Director, Mass Spectrometry Center, Janssen Research)

This presentation will highlight the performance merits of the latest Q-Tof technology for several platform product quality assays as well as innovative mass spec methods to elucidate issues that arise as molecules become more complex. Advances in MS instruments fill the gap to meet the analytical challenges of today’s biotherapeutics

Technology Driving Performance

• Martin R. Green (Scientific Fellow, Waters Corporation)

Q-ToF mass spectrometers are a well-established analytical tool utilized in a wide range of application areas. The high mass resolution, sensitivity, speed, and mass accuracy are essential in applications from cutting edge discovery to routine high throughput.The presentationdescribes modifications to a commercial Q-ToF MS to extend the dynamic range, improve the robustness and sensitivity, particularly for thermally labile compounds. These improvements result in a platform capable of bridging the gap between the qualitative performance of the Q-ToF and quantitative performance of the TQ instruments.

Quantitate with Absolute Power – Demonstration of our latest tandem quadrupole MS, the Xevo TQ Absolute

• Kate Wearden (Principal Product Manager, Waters Corporation)

Life at Waters

Life as an Early Career Scientist

• Owen Cornwell (Support Scientist, Waters Corporation)

Abstract coming...

Using an in-house UNIFI library with the ACQUITY RDa Detector to Determine Absolute Configurations of Amino Acids in Natural Products

• Michael Reccia (Ph.D. Chemistry Candidate, Simon Fraser University, Linington Lab)

The absolute configuration of the α-carbon (C-2) in amino acids is critical in the characterization of peptides. We developed an optimized analysis of Marfey’s method, a popular absolute configuration technique that compares retention times of derivatized hydrolysates to commercial ʟ,ᴅ standards. The improved analyses utilizes an in-house Waters UNIFI library in tandem with the ACQUITY RDa Detector, a high-resolution time-of-flight mass analyzer. The UNIFI library of FDVA-derivatized amino acids expedites the Marfey’s application by simultaneously acquiring data and processing samples for high-throughput determination of the α-carbon absolute configuration. This easily tailored workflow allows analysis of peptidic small molecules more efficiently.

Extraordinary Exploration with the SELECT SERIES

Sharing best kept secrets and unveiling new capabilities

• Emma Marsden-Edwards (Consulting Principal Product Manager, Waters Corporation)

Abstract coming...

Conformation-Specific Native Top-down Mass Spectrometry to Study Dynamic Protein Complexes and Conformations

• Hannah Britt (Ph.D. Graduate, University College London)

Native top-down mass spectrometry is a powerful tool for analysis of proteins and their complexes, characterizing backbone organization, interaction interfaces, solvent accessibility, and post-translational modifications. To date, such experiments have sampled across the entire conformational landscape, therefore the results reflect an average across multiple protein structures which cannot be delineated. Here, we combine electron capture dissociation with cyclic ion mobility-mass spectrometry to perform native top-down MS in a conformation-specific manner. Having successfully developed this method, it is now being applied to study protein conformation in cases of diseases known to be caused by misfolding or disorder of challenging proteins.

Desorption Electrospray Ionization - Mass Spectrometry Imaging and Cyclic Ion Mobility for the Study of Lipids in the Injured Brain

• Maureen A Kane (Associate Professor, Pharmaceutical Sciences; Executive Director, Mass Spectrometry Center, University of Maryland School of Pharmacy)

Desorption Electrospray Ionization (DESI) has emerged as a powerful ionization technique for lipid imaging. However, the complexity of lipid species, structures, and ionization make lipids difficult to study without any additional dimension of separation. An ion mobility separation effected by a cyclic ion mobility (cIM) device can declutter mass spectra from complex biological samples, separate isobaric and isomeric species and facilitate the identification of lipids. DESI-MSI for the study of lipids coupled to cIM separation will be discussed as well as the improvement from the new source (DESI-XS) and TOF analyser (Select Series).

LC-MS or Imaging: Closing the gap on tissue metabolomics

• Zoltan Takats (Professor of Analytical Chemistry, Imperial College London)

Abstract coming...