Strategies for Mycotoxins and Alkaloids Determination using LC and LC-MS/MS

Welcome to DAY 2 of the Waters Food Testing Forum 2022

Explore the latest technology and methodology solutions to help you more easily and efficiently deliver results for food testing.

• Discover new tools and solutions for multi-toxin detection and determination

• Learn how you can improve your analytical workflows 

• Panel discussion and Q&A chat box to answer your specific questions 

• Obtain valuable resources to show the practical application of Waters solutions, including application notes, case studies, and whitepapers.

AGENDA:

Session 1 (30 min): Improving the Robustness of LC-MS/MS Methods for Multi-Mycotoxins Analysis

• Nicola Dreolin (Waters)

Multi-toxin methods, using LC-MS/MS, are now commonly used for the determination of a wide range of regulated, “modified” and emerging mycotoxins in a variety of agricultural commodities and finished products. Modern, sensitive instruments can cope with the “dilute and shoot” approach that allows analysts to produce results in the most rapid manner. However, such continuous analysis of a wide range of different complex samples may necessitate implementing timely interventions for planned system maintenance or be faced with an unexpected loss of performance requiring immediate attention and often repeat analysis.

In this talk, you will learn how to implement different strategies for improving the robustness of your multi-toxins method, thus improving lab efficiency. We will present a toolkit of solutions tailored for the specific needs of your lab, starting from compliance testing of small groups of mycotoxins to meet regulatory criteria, to large multi-toxins methods.

Session 2 (30 min): A UPLC-MS/MS Method for the Determination of Pyrrolizidine Alkaloids in Botanicals

• Nicola Dreolin (Waters)

With the new Commission Regulation (EU) 2020/2040 entering into force in July 2022, all food companies producing specified food products for sale in the European Union must ensure that these products do not exceed maximum limits for the sum of 35 pyrrolizidine alkaloids (PAs).

Many laboratories have already implemented methods for the analysis of PAs.

In this talk, we will explore our solution based on UPLC-MS/MS for the quantification of 35 PAs in various food commodities. We will discuss method performance, including the separation of isomeric compounds and their N-oxide metabolites.

Product Spotlight (10 min): waters_connect for Quantitation Software.

Watch a short demonstration on how to rapidly process and review LC-MS/MS data post-acquisition of a validation batch of alkaloids, using the unique Exception Focused Review (XFR) functionality in the new waters_connect for Quantitation Software.

Panel Discussion (30 min): A Comprehensive View: Using Multi-Toxin Methods

• Professor Katrina Campbell (Food Security & Diagnostics, Institute for Global Food Security, Queen's University Belfast)
• Chiara Dall'Asta Ph.D. (Associate Professor (Dept of Food and Drugs), University of Parma)
• Michael Sulyok Ph.D. (Senior Researcher, University of Natural Resources and Life Sciences, Vienna)