Mitigating Analyte Interactions with Metal Surfaces in UHPLC for Pharmaceutical Applications

The non-specific binding of analytes to material surfaces is an inherent characteristic of conventional chromatographic columns and systems. This phenomenon leads to reduced sensitivity and sometimes poor peak shape of acidic molecules.

In this webinar, we will present a new, novel hybrid surface technology that provides a solution without the need for strong mobile phase additives, chelators, or lengthy passivation protocols. The chromatographic benefits provided when using columns and systems equipped with the MaxPeak™ High Performance Surfaces (HPS) technology will be demonstrated with antivirals, NSAIDs, cancer growth inhibitors, oligonucleotides, peptides, and glycans.

Key Learning Objectives:

• Understand how metal-analyte interactions can negatively impact chromatographic performance.

• Learn how to mitigate metal-analyte interactions using a novel surface technology.

• Explore applications: antivirals, NSAIDs, cancer growth inhibitors, oligonucleotides, peptides, and glycans.

Who Should Attend:

• Scientists who develop and/or manage UHPLC and HPLC methods in pharmaceutical laboratories.

• Lab managers leading teams involved with the analysis of small and large molecules

Presenter: Catharine Layton (Principal Scientist, Scientific Operations, Waters Corporation)

Catharine is a Principal Scientist on the Scientific Operations Platform Technologies Pharmaceutical Analysis Team at Waters Corporation located in Milford, MA. She works closely with technical experts to develop and provide the appropriate instrumentation and methodology for laboratories worldwide. She began her career in Biopharmaceutical Development R&D at a fast-paced contract research organization and drug development laboratory where she was responsible for designing analytical methods to characterize novel biological therapeutics formulated by key research organizations. Catharine later moved into large pharma and analytical instrument development, where she has dedicated many years to advancing and implementing analytical testing strategies and methodology.

Presenter: Martin Gilar, Ph.D. (Scientific Fellow, Separations R&D, Waters Corporation)

Martin received his Ph.D. in analytical chemistry from the Institute of Chemical Technology in Prague (1996). He spent postdoc years in Hybridon Inc. (1996-1998) and Northeastern University in Boston (1998) developing separation methods for antisense oligonucleotides and fraction collectors for DNA molecules. Since 1998 he has worked at Waters Corp. in Milford, Massachusetts, participating in the column, sample preparation, and instrument research.

Martin is a Scientific Fellow in the Separations R&D group at Waters Corporation. He has more than 30 years of experience in the separation sciences, including chromatography, electrophoresis, and mass spectrometry. His research interest is the analysis of biopolymers, peptides, and nucleic acids. He has published over 70 peer-reviewed papers. He is a recipient of the Chromatographic Society Jubilee medal in 2022.