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Key concepts of 2D-LC

RECORD | Already taken place We, 21.9.2022
Learn about the four modes of 2D-LC: heart-cutting, multiple heart-cutting, selective and full comprehensive, and find out how they relate conceptually.
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Agilent Technologies: Two-dimensional liquid chromatography (2D-LC): A tutorial focusing on best practice
Agilent Technologies: Two-dimensional liquid chromatography (2D-LC): A tutorial focusing on best practice

Two-dimensional liquid chromatography (2D-LC): A tutorial focusing on best practice

In recent years, 2D-LC has evolved from a niche technology to one that is in demand, especially when it comes to the analysis of complex samples, or hidden or co-eluting compounds. Whether you are starting out with 2D-LC or want to refresh your knowledge, this two-part webinar series will give you a comprehensive introduction to the technique, including best practices.

Session 1: Key concepts of 2D-LC

Learn about the four modes of 2D-LC: heart-cutting, multiple heart-cutting, selective and full comprehensive, and find out how they relate conceptually. Plus, get advice on how to choose complementary separations for the first and second dimension (RP, IEX, HILIC, etc.), how to optimize cycle time, and how to maximize second dimension sensitivity.

Who should attend?

New and experienced 2D-LC users, scientists, and lab managers or technical decision makers in pharma, biopharma, food, energy & chemicals, environmental, material, forensics, and life science research

Presenter: Dr. Dwight Stoll (Professor of Chemistry, Gustavus Adolphus College. St. Peter, MN, USA)

Dr. Dwight Stoll completed his undergraduate work at Minnesota State University, Mankato, in plant biology and biochemistry. Upon graduation in 1999, he took a job in industry as a research technician with ZirChrom Separations, Inc. For his graduate work, he studied with Professor Peter Carr in the Chemistry Department at the University of Minnesota from 2001–2007, where he worked on the development of fast, comprehensive two-dimensional liquid chromatography.

In the fall of 2008, Stoll was hired as an Assistant Professor in the Chemistry Department at Gustavus Adolphus College, where he mainly teaches quantitative and instrumental analysis courses. In 2014, he was promoted to Associate Professor, and in 2018, he was promoted to Professor. Currently, he is co-chair of the Chemistry Department. Active research projects in his laboratory touch upon most aspects of multi-dimensional separation methodologies, including optimization strategies, characterization of selectivity in reversed-phase HPLC, instrument development, and applications in biopharmaceutical analysis.

Stoll is the author or co-author of more than 62 peer-reviewed publications and three book chapters in the area of separation science, is a named co-inventor on four granted patents, and has instructed numerous short courses in two-dimensional liquid chromatography. In 2011, he was the recipient of LCGC’s Emerging Leader in Chromatography Award. In 2014, he added to The Analytical Scientist’s list of ‘Top 40 Under 40’ analytical scientists. In 2017, he received the Georges Guiochon Faculty Fellowship and an Agilent Technologies Thought Leader Award, which will support research in his laboratory on the development of 2D-LC methodologies for biopharmaceutical analysis.

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