Proteomics as a biological assay through sample multiplexing

Sample multiplexing using tandem mass tags (TMT) is emerging as a powerful approach for mass spectrometry-driven drug discovery and characterization of cellular signaling. However, sensitivity and throughput remain limiting factors toward achieving routine, full-proteome interrogation of the biological pathways.

This webcast will describe utilizing a modified Thermo Scientific™ Orbitrap™ Tribrid™ mass spectrometer system to evaluate multiplexed whole-proteomes coverage as a biological assay for drug discovery. Multidimensional comparisons of instrument features showed significant sensitivity improvement for TMT-based proteomics. We further utilized this technology to assess the protein abundances across the whole proteome, revealing protein abundance changes at superior depth and coverage.

You will learn:

• How to quantify more proteomic samples at lower concentrations
• How to achieve greater proteome coverage for peptides & PTMs
• How improved quantitation accuracy and precision enable the detection of low-abundant changes in protein abundance

Presenter: Dr. Steven P. Gygi (Department of Cell Biology, Harvard Medical School)

Steven Gygi, Ph.D., received his Ph.D. from the University of Utah in Pharmacology and Toxicology. In 2000, he moved to Harvard Medical School, Department of Cell Biology, where he focused on developing new technologies including systematic and proteome-wide measurements of protein properties, their expression levels, modification states, structure, localization, and interactions.

Moderator: Sarah Hiddleston (Science Journalist, Nature Research Custom Media)

Sarah Hiddleston is freelance journalist working with Nature Research Custom Media since 2015. Previously, Sarah worked for a decade in Madras (Chennai), India, specialising in health, pharmaceutical and environmental stories. Sarah holds an MA in Investigative Journalism from City University London, an MSc in Political Theory from the London School of Economics, and an undergraduate degree in History from the University of Cambridge, UK.