Fractionation
Peptide fractionation is important in proteomics workflows to reduce sample complexity and increase the separation efficiency. This leads to increased dynamic range of the identified peptides compared to conventional single-shot analysis.
The additional number of injections per fractionated sample is a good match with the short overhead time between injections on the Evosep One.
Optimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing
- Presenter: Claire Koenig (CPR)
Combination of isobaric labeling (TMTs) coupled with offline high-pH fractionation can maximize analysis depth in quantitative phosphoproteomics analysis. To investigate to what extent sample amount affect the sensitivity and the dynamic range of the analysis, we benchmark strategies for quantitative phosphoproteomics with limited peptide input amounts. Based on this research, we present a decision tree to guide phosphoproteomics users to find the best workflow, to reach optimal depth of coverage, based on experimental design and peptide availability.
More to be announced
