Recent Advances in Oligo Purity and Sequence Determination by LCMS
Oligonucleotides (oligos) have become fast-growing modalities in recent years. Along with the development of these candidates has come the increased need for robust analytical methods and easy-to-use data analysis workflows to characterize them. Critical quality attributes of oligos include their mass, purity, and sequence – each of which can be difficult, time consuming, and tedious. As such, software that supports these efforts can be of great value.
In this work, we present novel, automated, and integrated software to support these workflows using LC HRAM MS instruments. Purity and sequencing data are presented for a multitude of oligos including antisense, aptamer, and long synthetic samples.
Key Learning Objectives:
- Techniques for analyzing the critical quality attributes (CQAs) of oligos — including their mass, purity, and sequence
- How novel, automated, and integrated software can facilitate the analysis of oligo CQAs using LC–HRAM MS instruments.
- Purity and sequencing data for a multitude of oligos including antisense, aptamer, and long synthetic samples
Who Should Attend:
- Researchers analyzing the critical quality attributes (CQAs) of oligos — including their mass, purity, and sequence
- Chromatographers
- Researchers interested in using LC–HRAM MS instruments for determination of oligo mass, purity and sequence
Presenter: Peter Rye (Application Engineer Pre Sales, Agilent Technologies)
Presenter: Melissa O'Meara (Forensic Science Consultant, C&EN Media Group)
