Real-Time Analysis of Product Attributes in Downstream Processing of Viral Vectors

One of the key challenges during process development and production of biologics is the timely determination of essential product attributes. Rapid feedback of product-relevant characteristics simplifies and accelerates process development and scale-up, then helps ensure product yield and quality during full-scale manufacturing.

We present the use of real-time multi-angle light scattering (RT-MALS) as a process analytical technology to optimize process development of viral vectors. RT-MALS determines the biophysical properties size, molar mass and concentration of virions or nanoparticles. The application of RT-MALS in downstream processing of viral vectors is shown and discussed.

Key Learning Objectives:

• Introduction to real-time MALS (RT-MALS)
• How RT-MALS determines the biophysical properties size, molar mass and concentration of virions or nanoparticles
• Application of RT-MALS for viral vectors

Who Should Attend:

• Bio/pharmaceutical scientists responsible for process analytical technology (PAT), downstream processing, process development and production of biologics
• Process engineers seeking solutions for monitoring viral vectors or nanoparticle production processes

Presenter: Dr. Clara Pérez Peinado (Senior Scientist, Biophysics and Process Analytics Team, Janssen Vaccines & Prevention B.V)

Presenter: Dr. Felix Deluweit (Senior Application Scientist, Wyatt Technology Corp.)

Presenter: Kelly McSweeney (Contributing Editor, C&EN Media Group)