Speeding up method development - How Cyprotex have solved the analytical challenge of non-specific binding

An ongoing challenge when dealing with chromatographic separation is the presence of compounds that exhibit non-specific binding. Such compounds can exhibit poor peak shape, carryover, low sensitivity and cause an increase in back pressure and system clogging.

In high-throughput screening of compounds in drug discovery, non-specific binding can result in poor assay sensitivity and reproducibility, prolonged data analysis and complicated method development.

In this webinar, Fiona Liddicoat and Jack Corrigan from Cyprotex, a leading ADME-Tox service provider, demonstrate how ACQUITY Premier column and system using MaxPeak High Performance Surfaces (HPS) technology can address the challenge of non-specific binding for small molecules and peptides. The benefits of this new technology include increased sensitivity, improved peak shape and reduced carryover.

This webinar focuses on:

• The application of MaxPeak HPS technology for enhanced chromatographic performance of pharmaceutical compounds in UPLC-MS
• The comparison of chromatographic performances of traditional stainless steel UPLC technology and the ACQUITY Premier solution

Key learnings:

• Learn how new chromatographic technology:

  • significantly reduces method development turnaround
  • allows rapid diagnosis of non-specific binding
  • eliminates passivation requirement
  • applies to ADME screening

• Discover the benefit of ACQUITY Premier column and system in:

  • eliminating non-specific binding
  • improving peak shape
  • increasing sensitivity
  • reducing carryover

• Increase your knowledge in pharmaceutical compounds screening with UPLC-MS

Presenter: Fiona Liddicoat (Associate Principal Scientist, Cyprotex)

Fiona is an Associate Principal Scientist at Cyprotex leading high throughput analytical method development. Fiona graduated from University of Strathclyde with an analytical chemistry master’s degree before gaining experience in the pharmaceutical drug discovery industry. Fiona went on to join Waters as an applications chemist specialising in pharmaceutical analysis before joining Cyprotex in 2021.

Presenter: Jack Corrigan (Associate Scientist, Cyprotex)

An enthusiastic Analytical Chemist, Jack holds a first class master's degree in Chemistry. He has gained significant experience in the upkeep and use of a wide range of UPLC-MS techniques in his 2 years as an Associate Scientist at Cyprotex. As someone who understands the value of cooperation, Jack has been involved previous collaborations with Waters producing both posters and presentations showcased at various events including the DMDG early careers conference.