Single cell proteomics, from sample preparation to results processing

Single cell proteomics is growing as an analytical tool, delivering in-depth information of molecular processes occurring at the cellular level. The release of the timsTOF SCP in June 2021 opened the gate to label-free analysis of single cells.

This approach removes the needs of labelling and their associated costs and bias. Removing this step is not sufficient to handle all the challenges associated with the extremely diluted sample preparation, handling and processing.

In this live from the lab, we will present and demonstrate the use of a complete SCP ecosystem allowing to address the sensitivity and robustness issues at all stages of the analytical process, starting from single cell sorting and digestion down to the data analysis, considering sample transfer, separation, dia-PASEF® acquisition and real-time data processing.