Are Biphenyl Phases really the new C18?
When approaching development of a new HPLC method, one of the most forgone conclusions is that to start off, you grab a C18 column and work forwards from there. Whilst C18 is a great stationary phase, and is heavily relied on all around the world, this webinar aims to offer an alternative 'generic' phase you may want to consider.
In this webinar we'll discuss the retention profile of C18 columns, and consider the differences which modified C18 phases can offer. We’ll then consider the retention mechanism of a biphenyl stationary phase and contrast between the two. After this, we’ll look at some practical tips for getting the most from biphenyl columns, and compare a few practical examples of chromatography produced using both a C18 and biphenyl phase.
This webinar aims to:
- Provide an overview of the retention mechanisms at play with various C18 column types
- Discuss how biphenyl columns retain, where they differ from C18, and how to benefit from these differences
- Teach the best practices for successful utilization of biphenyl columns in LC methods
- Demonstrate examples of biphenyl chromatography in applied solutions
