RapidFire affords extreme versatility in one platform for detecting reaction analytes across a diverse group of enzyme targets

This presentation will focus on our use of a RapidFire 400 High Throughput Mass Spectrometry system coupled to a triple quadrupole mass spectrometer, to build and implement a single platform for the rapid biochemical characterization of multiple biological enzyme targets.

Because of the high sample throughput compared to traditional LC-MS/MS, we can comprehensively characterize the activity of multiple enzymes across a variety of substrates including biologically relevant substrates (e.g. oligonucleosomes) and tailor our Medicinal Chemistry small molecule compound design efforts to target the enzyme activity of interest. Employing a single platform with internal redundancy, enables us to both quickly and confidently develop suites of assays within and across multiple enzyme classes, without many of the limitations present with other technologies.

Presenter: Patrick J. Bingham (Senior Scientist, Pfizer)