PRINCIPLES AND PRACTICAL STEPS IN DEVELOPING REVERSE-PHASE PEPTIDE SEPARATIONS

As a continuation of our Chromatography of Bioseparations 101 series, we bring to you 2 NEW SESSIONS in our Chromatography of Bioseparations 102 webinar series! Join us live for our new parts 6 and 7. If you haven't had an opportunity to register for Parts 1-5, feel free to regster and listen to them on-demand now.

These new webinars will focus on the analytical separation of biomolecules such as peptides and amino acids.

Reverse-phase chromatography is the predominant technique used for the analyses and or purification of chemically synthesized peptides or peptides generated by the enzymatic digestion of proteins (i.e., peptide mapping). It is advantageous to have a simplified step-by-step approach to develop a method that can be applied to chemically synthesized peptides or protein digests.

Amino acid analysis is required in several different areas of research and is a fundamental tool in various product analytical activities. Due to the varied properties of amino acids (i.e., acidic, basic, etc.), the separation and detection represents an analytical challenge and depending on throughput, sample preparation can be laborious.

Who should attend?

• Those interested in a refresher on foundational principles and tips of reverse phase separations of peptides.
• Those interested in how to select columns and mobile phases that are optimal for peptide and amino acid workflows.
• Those looking for an overview of industry advancements and new offerings.

PART 6 | June 20th, 2023 | 10:30AM – 12:00PM EDT

PRINCIPLES AND PRACTICAL STEPS IN DEVELOPING REVERSE-PHASE PEPTIDE SEPARATIONS

• Learn how column chemistry and mobile phase affects peptide separation and selectivity.
• Review the factors that synergistically work to obtain desired resolution of peptides contained in a complex mixture.

Presenter: Bill Warren (Consulting Product Manager – Consumables and Lab Automation, Waters Corporation)

Bill received his MS in Microbiology and Immunology from the University of Louisville School of Medicine. In 1986, joined Waters Corporation as an senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids. Following four years as a field based technical sales representative for Waters, Bill enjoyed a tenure as Manager, Core Product Evaluation Laboratory within the Waters Worldwide Marketing Organization. Bill currently is a Principal Bioseparation Columns Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of proteins and peptides.