New Developments and Tips for Oligonucleotide Analyses
Involved in developing oligonucleotide therapeutics? Oligonucleotides have attracted increasing attention in the development of direct therapeutic agents against a wide range of disease conditions.
The analysis of oligonucleotide therapeutics requires robust and accurate analytical characterization to confirm identity, and to determine purity, quality, and strength.
During this webinar, Thermo Scientific’s Ken Cook will examine developments and tips for:
Column chemistry to fully sequence a 100mer by LCMS/MS and HCD fragmentation
A method to monitor IVT reactions by rapid UHPLC
Observe disterio-isomer profiles in siRNA and ASO RNA
Change separation selectivity in ion exchange and ion pair reverse phase chromatography of oligonucleotides with eluent choice and oligonucleotides with pH
Methods for strand ratio determination with double stranded oligonucleotides
Thermo Scientific has over 20 years of innovations and publications around the separation of oligonucleotides, please join our webinar to learn how the DNAPac family of Ion Exchange and Reversed Phase columns can optimize and enhance your nucleic acid separations. The session will finish with a short Q&A and include a live chat with an expert.
Presenter: Dr. Ken Cook
Dr. Ken Cook began his career in academia as a Biochemistry lecturer focused on protein chemistry and metabolism. Today, with well over 30 years’ industry experience working for Dionex, then Thermo Fisher Scientific, his vast knowledge supporting and developing solutions within chromatography brand him a bio-separations expert.
Currently, his work involves the support of Bio-Pharmaceutical applications to characterise protein and oligonucleotide-based bio-therapeutics. This involves ongoing collaborations with the Biopharmaceutical industry and academia. Methods for the characterisation of therapeutic proteins include peptide mapping by LC/HRMS. Developments in charged variant analysis including novel coupling to MS, Glycan analysis, Aggregate analysis and on-line process monitoring. Gene therapy characterisation is a growing interest which has had much success.
Given his immense experience, it is an honour to have Ken share his knowledge.
