Cost-Effective Oligonucleotide Purification and Scaling without HFIP
Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
PART 6 | Cost-Effective Oligonucleotide Purification and Scaling without HFIP
Chemists face hurdles to achieving cost-effective laboratory scale purification of oligonucleotides; coping with expensive HFIP ion pairing mobile phases, and scaling from analytical UPLC methods to semi-preparative scale separations is not as simple as it seems. Here we present the impact of several techniques in the purification of a 20-mer oligo in the absence of HFIP.
Learning Objectives:
- Recognize alternative separations chemistry for purification
- Balance purity and recovery requirements
- Learn purification techniques to employ in the laboratory
Presenter: Andrew Leightner (Market Development Manager – Americas Field Marketing, Waters Corporation)
Andy Leightner has over 10 years of experience serving the pharma market from contract research organizations. He specialized in method development and validation leveraging LC, GC, and mass spectrometry to meet complex client needs. He supports pharmaceutical and biopharmaceutical discovery and development in the Americas for Waters Corporation. Andy received a bachelors degree in biology from the University of Wisconsin-Madison.
