Polar Analytes C18 Didn’t Work, Now What

C18 is the most common column for HPLC but what if it doesn’t work for your sample. Very polar compounds have poor retention in reversed phase and can be challenging to separate.

In this talk, we will discuss changes to your reversed phase conditions that can help. We’ll also look at the benefits of choosing a more polar stationary phase. If reversed phase doesn’t work you may need to explore other types of chromatography, like HILIC or Ion Pair Chromatography.

Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)

Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.