Making the Leap: Small Molecules to Biomolecules
Whether you’re currently working with biomolecules or are making a transition from working with small molecules to biotherapeutics you will find that size does make a difference in the decisions we make as we’re developing a method.
Some characteristics of biomolecules can make their separations both different from and more challenging than small molecule separations. Of the many different HPLC separation mechanisms and column types, reverse phase is commonly used for both small molecules and biomolecules. This presentation will look at what we typically think about for small molecules and how working with biomolecules may be similar or in some cases quite different.
Areas we’ll discuss are:
Method development parameters; similarities and differences
Column selection
The matrix and sample prep
Instrument considerations
Tips to help identify why your separation doesn’t look like you thought it would
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
