Updating Old Methods, Is the Gain Worth the Pain?

Improving an existing method is an opportunity to save time, money and sample.

Whether you’re developing a new method or updating an older one, there are several questions to consider, including:

• Is the goal to reduce analysis time and/or improve resolution?

• Is the method isocratic or gradient?

• What HPLC parameters will be key to success?

• What are the column options and will they work with your instrumentation?

• Modernizing a legacy method, whether it’s a reference method from the USP, AOAC, or EPA or an internal one, can simplify testing and improve accuracy, ruggedness, and sample throughput.

Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)

Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.